GB —2010 National food safety standard Food microbiological examination:Aerobic plate count Aerobic plate count Food test sample is treated under certain conditions after (. medium, culture temperature and culture time) culture, resulting per g (mL) The total number of microbial colonies formed seized samples 1 Equipment and Materials In addition to the microbiology laboratory and training routine sterilization equipment, other equipment and materials as follows: incubator: 36 ℃± 1 ℃, 30 ℃± 1 ℃. Refrigerator: 2 ℃~ 5 ℃. thermostatic water bath: 46 ℃± 1 ℃ Balance: a sense of the amount of g. homogenizer. oscillators. Sterile pipette: 1 mL ( mL with scale), 10 mL ( mL with scale) or pipette and tip. sterile conical flask: capacity 250 mL, 500 mL. sterile Petri dish: diameter 90 mm. pH meter or pH colorimetric tubes or precision pH test paper. magnifier and / or colony counter. 2 media and reagents plate count agar medium: See Appendix A, . phosphate buffer: See Appendix A . Sterile saline: See Appendix A 3 Inspection Program The total number of colonies inspection program in Figure 1 Test sample 25 g (mL) sample +225 mL diluent, homogeneous Report Calculate the total number of colonies 10-fold serial dilutions Choose two or three suitable dilution liquid sample un
