HPLC高效液相色谱测定海尔福口服液中甘草酸含量 韦浪杨日芳李桃山王娜梁永赞何胜张树球(通讯作者) (右江民族医学院重金属与氟砷毒物研究实验室广西百色 533000) 【摘要】目的采用高效液相色谱(HPLC)测定海尔福口服液中甘草酸含量,并与甘草药材提取液进行对比分析。方法采用高效液相色谱法,色谱柱: Acclaim ? 120 C18 5 μm (×250mm) 流动相: 甲醇-水-冰乙酸(71:28:1) 波长: 250nm 流速:。结果(1)± mg/g生药;(2)±;(3)±;(4)海尔福口服液中甘草酸含量为 ± ;(5)±;各样品甘草酸含量比较,p<,差异均有统计学意义;%,进样20μ—,相关系数为R=,精密度RSD%=%。结论该法测定准确, 线性关系好,可用于海尔福口服液质量控制. 【关键词】高效液相色谱(HPLC) 海尔福口服液甘草酸 【中图分类号】 【文献标识码】A 【文章编号】2095-1752(2013)32-0095-02 Determination of Glycyrrhizic acid content in Hai er fu oral liquid by HPLC Wei lang Yang Ri-fang Li Tao-shan Wang na Liang Yong-zan Zhang Shu-qiu (Heavy metals and fluoride arsenic poison Research Laboratory, Youjiang Medical College For Nationalities, Baise,Guangxi,533000) 【Abstract】 Objective Determination of Glycyrrhizic acid content in Hai er fu oral liquid by HPLC, parative analysis with Licorice extract. Method Using high-performance liquid chromatography, the determination was performed on an Acclaim ? 120 C18 5 μm (×250mm) column, with the mobile phase was methanol - water -glacial acetic acid (71:28:1) at a flow rate of , and the detection wavelength was