广西砷暴露人群血液生物标志物的初探
黄小琪1 陈晶1 林洪升2 黄盼柳3 林英辉2 通讯作者广西中医药大学第一附属医院 ,, 530023 基金项目:广西科学研究与技术开发计划课题
【摘要】目的探讨金属硫蛋白(Metallothionein,MT)、嘌呤核苷磷酸化酶(PurineNucleosidePhosphorylase,PNP)、O-GlcAc糖基转移酶(O-lingedGlcActransferase,OGT)mRNA 的表达水平作为职业性砷暴露人群早期生物学指标的意义方法用原子荧光分光光度计检测尿砷含量,Real-timePCR 方法检测血样中MT1a、MT2a、PNP和OGT的mRNA ,MT1a、MT2a、PNP、和OGT mRNA 表达水平高于对照组. 结论 MT1a、MT2a、PNP、和OGT可作为广西职业性砷暴露人群早期检测的生物学标志. 【关键词】砷暴露;金属硫蛋白;嘌呤核苷磷酸化酶;O-GlcAc糖基转移酶;ThestudyofpopulationwitharsenicexposurebloodbiomarkerinGuangxi HUANGXiao-qi1,CHENJing1,LIN Hong-sheng2,LIN Ying-hui2△(1 Departmentof MolecularBiologyLaboratory,2DepartmentofLaboratory,TheFirstAffiliated HospitalofGuangxiUniversityofChineseMedicine,NanGning 530023,China??E-mail:******@163??com )【Abstract】AIM:ToexploretheexpressionofMetallothionein(MT)、PurineNucleosidePhosphorylase(PNP)、O-lingedGlcActransferase(OGT)mRNAwhichsignificanceofearlybiologicalmarkersinarsenicexposurepopulation?? Methods:TheuricarsenicwasdeterminedbyAtomicfluorescencespectrophotomeGter??ThemRNAexpressionofMT1a、MT2a、PNP、
OGTwasevaluatedbyquantitativereal-timePCR??Results:Theconcentrationofuricarsenicofcasegroupishigherthancontrolgroup??ThemRNAexpressionofMT1a,MT2a,PNPandOGTarehigherwithcasegroup?? CONCULUSION:MT1a,MT2a,PNP,andOGT【cKoeuyldwobredesa】upationalexposuretoarsenicinGuangxi?? Arsenicexposure;Metallothionein;NucleosidePhosp
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