白藜芦醇干预对已退变椎间盘终板软骨细胞SIRT1表达的影响.pdf

白藜芦醇干预对已退变椎间盘终板软骨细胞SIRT1表达的影响.pdf!!!
SIRTl
1胡侦明沈皆亮甘强郝杰厍煲娇拼笱Ц绞舻谝灰皆汗强疲厍
R猐 2l(slRTl)
P2sIRTl
mRNADMs0sIRTlmRNA(P>005)125moLL
用 h50moLL12hsIRTlmRNA(P<005)白藜芦醇干
预可促进退变椎间盘终板软骨细胞表达鞍准癿腋米饔贸氏峙ǘ取⑹。
丶蔧白藜芦醇;浓度;时间;退变;软骨终板细胞;籗
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concentration-dependent inductiofSIRTlhdegenemvedplatechondmcytes
ZHONG Xiao-MingHUZhen-MingSHENJieLiangL
Depanment orthopaedicstheFirst Ⅱ HpitalChongqingMedical 璐珻 4016China
【】 obiective explore the efkct dif!f'erent and Resveratrol the
SIRTl human chondmcvtesMethols Denemtive hanrested elder
patient 琣. Passage 2 DEC 痚 and .
specvelvand SIRTl protein and the end stimulationRlllts the —
SIRTl and SIRTl gmuptherewas significant . Resveratrol
125moLLand hoursResveratml umoLL hours could stimulate the expressions SIRTl protein and mRNA(P<
. ConclioResveratrolintervention significantlv stimulate the expressions protein and human DEC a
concentmtionandtimedependent % has SIRTl protein .
Keywords ResveratmlconcentrationDurationDegenerationEndplatechondmcytessIRTlsIRTl
椎间盘退变时,终板退变导致的使髓核营养供应减少及代 USA)SIRT(AbcamusA)cOLA2dlaggreean
谢产物排出减少,是促进髓核退变的重要因素。白藜芦醇 cmzUSA)HRP(GenScriptUSA)RNA
(REs)PCR()JY300C
REs()uVPcDs8000(uVPusA)
2l(sIRTl)sIRrllFTc3000 — PcR(Funglyn琧。
抵抗各种应激及炎症反应的作用,是目前发现的最好的天然方法
せ罴痢D壳埃腥狈对退变椎间盘细胞抗凋亡及 121人退变椎间盘终板软骨细胞的分离与培养退变椎间
抑制退变方面的研究。本研究探讨白藜芦醇干预对退变椎间盘终板软骨细胞获取自老年腰椎间盘突出症患者手术时刮除
SIRTl664726783
研究提供参考。例,女痪跚癕及术后病