免疫-白细胞分化抗原.ppt

Chapter VII Leukocyte differentiation antigen and Adhesion molecule
When T cell meets DC or B cell ………
LDA,CD,CAM
Functional molecule and human leukocyte differentiation antigen on immunocyte surface
3. Clinical application of CD and adhesion molecule as well as their monoclonal antibody
Cell Surface Molecules and HLDA
Surface Molecules of Immune cells
Receptors: TCR, BCR, PRR, CKR, CR
MHC: MHCⅠⅡ
Co-stimulatory Molecules: CD28
Adhesion Molecules: integrin,selectin
Cell Surface Molecules
Leukocyte differentiation antigen(LDA) :The cell surface markers which express or disappear on the different leukocytes in the different stage of differentiation and activation.
LDA
Expressed on leukocyte, erythrocyte and megakaryocytes or thrombocytes
Other non-hematopoietic cells
Trans-membrane protein or glycoprotein
Some are anchored on plasma membrane with GPI conjunction
Immunoglobulin superfamily
Cytokine receptor family
Type C lectin superfamily
Integrin family
TNF superfamily
TNF receptor superfamily
Classification of LDA
Cell Surface Molecules
CD (cluster of differentiation) : Cell surface molecules of leucocytes that are distinguishable with monoclonal antibodies as an immunologic marker.
HCDM(Human cell differentiation molecules)
Rules for assigning a CD number
at least one Workshop—characterised antibody and good molecular data
the antigen is expressed on the surface of cells involved in immune reactions
the antibodies react with primary cells (not just transfectants or binant protein)
the antibody is available.