培养基成分对黔白大白菜杂交种小孢子胚诱导的影响.pdf

贵州农业科学 2007,35(6):24~25
GuizhouAgricultura1 Sciences
[文章编号]1001—3601(2007)06—0365—0024—02
培养基成分对黔白大白菜杂交种小孢子胚诱导的影响
赵大芹,陶莲,张朝君,毛堂芬,徐涵。
(,贵卅I省园艺工程技术研究中心,贵卅I贵阳 550006;,
贵州贵阳 550006;,图卢兹 31300)
[摘要]以 2个黔白大白菜杂交组合为材料,进行游离小孢子培养,探讨了培养基成分对小孢子成胚率的影响结果表
明:NLN培养基添加 13 蔗糖的培养效果最好;添加 6-BA和 NAA的培养基显著提高了小孢子胚的
诱导率和成熟胚的比例;NLN-13液体培养基中添加适量活性碳可明显促进小孢子胚状体的形成,对胚胎发育的同步性也有
很好的作用。
[关键词]大白菜;小孢子培养;培养基成分;胚状体;诱导率
[中图分类号]$ [文献标识码]A
The Effect of position on Embryoid Differentiation Rate of
Microspores ofQianbai Chinese Cabbage binations
ZHA0Daqin ,TAOLian ,ZHANGChaojun ,MAOTangfen。,XU Han。
( Horticultural Institute, Guizhou Horticultural Engineering Research Center, Guiyang, Guizhou
550006;-technologicalInstitute,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,
China; Research Institute,Toulouse31300,France)
Abstract:The microspores fromtwo binations ofQianbai Chinese cabbage were thecultured toinvestigate
effectof position on the embryoid differentiation resultsshowed that the best induced was effect from NLN
media4-13 sucrose,theNLN media4- 6-BA4- mg/L NAAimproved significantly embryoidthe induced rate
and ofmatureratio embryos and NLN一13 1iquidmedia4-rationa1 active carbon obviously promotedthe embryoid formation and
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