款冬花止咳有效部位和紫菀配伍“相须”部位的研究论文.doc

款冬花止咳有效部位和紫菀配伍“相须”部位的研究论文.doc款冬花止咳有效部位和紫菀配伍“相须”部位的研究论文
张巧真, 张燕, 张勉*, 黄芳, 窦昌贵, 金育
【摘要】目的观察款冬花及其有效部位与紫菀不同部位配伍后对止咳作用的影响,寻找款冬花与紫菀配伍中紫菀止咳的相须部位。方法通过小鼠氨水引咳实验观察款冬花、款冬花与紫菀配伍的止咳作用。结果款冬花、款冬花与紫菀的配伍均能减少氨水引咳小鼠的咳嗽次数,其中款冬花水提物及其90%醇沉部位止咳效果最好;配伍实验显示,紫菀30%醇提物及其90%醇溶部位与款冬并配伍后,对氨水引咳小鼠的止咳作用最为明显。结论款冬花水提物的止咳效果优于乙醇提取物;水提物中90%醇沉部位效果最好,为款冬花止咳作用的有效部位。紫菀30%乙醇提取物对款冬花止咳的增效作用优于其他溶剂;而其90%醇溶部位与款冬花配伍后止咳作用的增强效果最为显著,确定该部位为配伍中紫菀的相须部位。
【关键词】款冬花有效部位紫菀配伍相须部位
Abstract:ObjectiveTo search the antitussive part of Flos Farfarae (FF) and the reinforcement part of Radix Asteris (RA) in the paired drug (pound drug) of FF and RA. MethodsEvaluating the antitussive effect by cough times of mice caused by ammonia. ResultsBoth FF and the pair-drug of FF and RA can reduce the cough times of mice caused by ammonia .The aqueous extract (AE) of FF and its part of insoluble in 90% ethanol (90%insol-part) shoore effective than its ethanol extracts, and the 90%insol-part of FF is the effective antitussive part. In pound drugs, the 30%-EE and the 90%sol-part of RA can greatly reinforce the antitussive effect of FF. According to the patibility theory of traditional Chinese medicine, the 90%sol-part of RA should be the promotional part to FF in the paired drug.
Key otional part

款冬花为菊科植物款冬Tussilago farfara ,味辛、 g量连续灌胃给药3 d,空白对照组给予等体积的蒸馏水(滴加1滴吐温80),咳必清作为阳性对照。末次给药1h后,分别迅速向500ml宽口瓶内喷入等浓度等体积的浓氨水,密封,饱和2 min后将小鼠单只迅速放入其中。观察从放入小鼠到首次出现咳嗽的时间(潜伏期)及小