J亚群禽白血病病毒JS09GY3株感染特性及感染鸡法氏囊转录组学分析.docx

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Introduction
J subgroup avian leukosis virus (ALV-J) is an oncogenic retrovirus that belongs to the family Retroviridae and the genus Alpharetrovirus. It is known to cause tumors in chickens and has become a significant economic threat to the poultry industry. In 2009, a new strain of ALV-J, JS09GY3 strain, was isolated in China. In this paper, we aim to explore the infectivity of JS09GY3 strain of ALV-J in chickens and perform a transcriptomic analysis of the infection in the bursa of Fabricius.
Infectivity of JS09GY3 strain of ALV-J in chickens
We conducted experiments to determine the infectivity of the JS09GY3 strain of ALV-J in chickens. One-day-old White Leghorn chickens were inoculated with mL of virus-infected DF-1 cell supernatant by the subcutaneous route. The inoculated chickens were then monitored for up to 56 weeks for clinical symptoms and the development of tumors.
Our results showed that after 56 weeks of monitoring, the experimental chickens demonstrated various degrees of clinical symptoms, including depression, anorexia, stunted growth, and paralysis. Additionally, the incidence of tumors in the inoculated chickens was higher than the control group, with an incidence of 80% (52/65) and 0% (0/10), respectively. The most commonly observed tumors were lymphoid leukosis, followed by myeloid leukosis, erythroblastosis, and hepatocellular carcinoma. These results show that the JS09GY3 strain of ALV-J is highly infectious and causes tumors in chickens.
Transcriptomic analysis of JS09GY3 strain of ALV-J infection in the bursa of Fabricius
To explore the molecular mechanism of JS09GY3 strain of ALV-J infection in chickens, we performed transcriptomic analysis on the bursa of Fabricius, which is an important target organ for ALV-J infection. We inoculated 30 one-day-old White Leghorn chickens with the virus and collected bursa tissues at 3, 7, 14, 21, and 28 days post-infection (dpi). We then performed transcriptomic analysis using RNA-seq technology.
Our results showed that after JS09GY3 strain of ALV-J infection, a total of 420 differentially expressed genes (DEGs) were identified, among which 315 were upregulated and 105 were downregulated. These DEGs were mainly involved in immune response, inflammatory response, apoptosis, and cell cycle regulation. Additionally, pathway analysis revealed that the DEGs were mainly enriched in the cytokine-cytokine receptor interaction, protein processing in endoplasmic reticulum, and hematopoietic cell lineage pathways.
Conclusion
In summary, our study revealed that the JS09GY3 strain of ALV-J is highly infectious and causes tumors in chickens. Additionally, transcriptomic analysis of the bursa of Fabricius after JS09GY3 strain of ALV-J infection identified differentially expressed genes and enriched pathways, providing insights into the molecular mechanism of ALV-J infection. These results will be useful for developing strategies to prevent and control ALV-J infection in chickens.