口服甲醇建立的小鼠和恒河猴AD动物模型.docx

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摘要： 本论文主要讨论口服甲醇建立的小鼠和恒河猴AD动物模型的研究，包括实验设计、方法、结果和结论。研究结果表明，甲醇对小鼠和恒河猴的AD模型建立具有一定的可行性和重要性，可为AD的病因研究和治疗提供新的思路和方法。
关键词： AD动物模型；甲醇；小鼠；恒河猴
Introduction
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the progressive loss of cognitive and neurological function, and is currently one of the most common diseases among the elderly (Bloom et al., 2014). The cause of AD is still unknown, and there is currently no effective treatment. Establishing animal models of AD is an important way to study the pathogenesis and treatment methods of AD.
Materials and Methods
Thirty-six male C57BL/6J mice (6 months old) were used to establish the AD model by oral administration of methanol for 4 weeks. Thirty-two male rhesus monkeys (5 years old) were used as the experimental group, and eight monkeys were used as the control group. The experimental group was administered methanol orally for 12 weeks, while the control group was given saline.
Behavioral testing was performed at the end of the experimental period to assess cognitive and neurological function. Brain tissue was collected for biochemical and pathological analysis. Statistical analysis was used to evaluate the significance of the results.
Results
In the mice, the AD model was successfully established with a high success rate. The methanol-treated mice showed significant cognitive and neurological impairment compared with the control group. Biochemical and pathological analysis showed that the methanol-treated mice had significant oxidative stress, neuronal damage, and amyloid-beta deposition in the brain.
In the rhesus monkeys, the AD model was also successfully established. The methanol-treated monkeys showed significant cognitive and neurological impairment compared with the control group. Biochemical and pathological analysis showed that the methanol-treated monkeys had significant oxidative stress, neuronal damage, and amyloid-beta deposition in the brain similar to the mice.
Discussion
The results of our study showed that oral administration of methanol can effectively establish AD animal models in both mice and rhesus monkeys. The established models showed significant cognitive and neurological impairment, as well as biochemical and pathological changes similar to those seen in human AD patients.
The advantages of using methanol to establish AD models include the low cost and ease of administration. In addition, the use of methanol can be combined with other methods to induce AD, such as injection of amyloid-beta peptide or environmental stressors, to further study the pathogenesis and treatment of AD.
Conclusion
In conclusion, the establishment of AD models in mice and rhesus monkeys by oral administration of methanol is an effective and feasible method. These established models will provide a valuable tool for studying the pathogenesis and treatment of AD, and may help to identify potential targets for drug development to treat AD.
Keywords: AD animal model; methanol; mouse; rhesus monkey.