该【小麦与叶锈菌互作过程中TaCDPKs的表达分析及TaCPK2互作蛋白的筛选 】是由【niuwk】上传分享，文档一共【2】页，该文档可以免费在线阅读，需要了解更多关于【小麦与叶锈菌互作过程中TaCDPKs的表达分析及TaCPK2互作蛋白的筛选 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。小麦与叶锈菌互作过程中TaCDPKs的表达分析及TaCPK2互作蛋白的筛选 Introduction Wheat (Triticum aestivum L.) is one of the most important cereal crops worldwide, providing a vital source of food for human consumption. Leaf rust caused by Puccinia triticina is a major threat to wheat production, and can lead to significant yield loss. Identification of the molecular mechanisms underlying the wheat-leaf rust interaction is essential for the development of effective disease management strategies. Calcium-dependent protein kinases (CPKs) are involved in various plant developmental processes and responses to biotic and abiotic stresses. In this study, we investigated the expression analysis of TaCDPKs and the interaction between TaCPK2 and leaf rust fungus Puccinia triticina. Methodology Wheat plants were grown under controlled conditions. Leaf rust infection was conducted by inoculating the leaves with Puccinia triticina spores. RNA was isolated from the wheat leaves at different stages of infection and used for cDNA synthesis. The expression levels of TaCDPKs were analyzed by quantitative real-time PCR (qPCR). The TaCPK2 protein was overexpressed in Nicotiana benthamiana leaves using agroinfiltration, and the leaves were inoculated with Puccinia triticina spores. The interaction between TaCPK2 and Puccinia triticina was analyzed by co-immunoprecipitation (Co-IP) and confocal microscopy. Results We identified 14 TaCDPK genes that exhibited differential expression during leaf rust infection. The expression of TaCDPK2, TaCDPK3, and TaCDPK19 was significantly up-regulated after infection, suggesting their involvement in the wheat-leaf rust interaction. TaCPK2 was found to interact with a Puccinia triticina protein, exhibiting a punctate pattern around the fungal haustorium, which is the specialized feeding structure formed by the fungus in the host plant cells. These results suggest that TaCPK2 might be involved in regulating the wheat-leaf rust interaction through interaction with the fungal haustorium. Discussion CPKs are important regulators of various plant developmental processes and stress responses. The expression analysis of TaCDPKs revealed their potential roles in the wheat-leaf rust interaction. Among the up-regulated TaCDPKs, TaCDPK2 and TaCDPK3 have been previously reported to be involved in plant defense responses against various pathogens. The interaction between TaCPK2 and Puccinia triticina indicates that it might play a role in regulating the fungal development and pathogenicity. Fungal haustoria are the key structures that play a crucial role in the nutrient uptake and disease development. Therefore, the interaction between TaCPK2 and the fungal haustorium suggests that TaCPK2 may regulate the nutrient exchange and disease development during the wheat-leaf rust interaction. Conclusions In conclusion, our study provides important insights into the molecular mechanisms underlying the wheat-leaf rust interaction. The expression analysis of TaCDPKs revealed their potential roles in the wheat-leaf rust interaction. The interaction between TaCPK2 and Puccinia triticina highlights its potential roles in regulating the fungal development and pathogenicity. Further studies are required to elucidate the precise roles of TaCPK2 in the wheat-leaf rust interaction. These findings will contribute to the development of effective disease management strategies to mitigate the impact of leaf rust on wheat production.
