乙烯调控双孢蘑菇采后成熟的分子机制研究.docx

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Title: Molecular Mechanisms Underlying the Regulation of Post-harvest Ripening in Agaricus bisporus by Ethylene
Abstract:
Agaricus bisporus, commonly known as white button mushroom, is a popular edible mushroom worldwide. The post-harvest ripening of A. bisporus is a critical process which significantly affects its nutritional quality, shelf-life, and sensory attributes. Ethylene, a plant hormone known to play a key role in various developmental processes, has been shown to regulate fruit ripening in many plants. However, the molecular mechanisms underlying the regulation of post-harvest ripening in A. bisporus by ethylene remain poorly understood. In this study, we aimed to explore the molecular pathways involved in ethylene-mediated post-harvest ripening in A. bisporus.
Introduction:
Agaricus bisporus is one of the most commercially cultivated edible mushrooms globally. Its nutritional value, unique taste, and versatile culinary applications contribute to its high demand. However, the post-harvest ripening of A. bisporus is a complex process influenced by various factors, including temperature, humidity, and ethylene. Ethylene, a gaseous plant hormone, has been implicated in ripening and senescence processes in a diverse range of fruits and vegetables. Understanding the molecular mechanisms by which ethylene regulates post-harvest ripening in A. bisporus is essential for optimizing storage and enhancing the shelf-life of this economically important mushroom.
Methods:
To investigate the molecular mechanisms involved in ethylene-mediated post-harvest ripening in A. bisporus, we utilized a combination of molecular biology techniques and transcriptomic analysis. Freshly harvested A. bisporus samples were exposed to different concentrations of ethylene, and samples were collected at several time points during the ripening process. RNA sequencing was performed to identify differentially expressed genes associated with ethylene response and ripening. Functional annotation and pathway enrichment analysis were conducted to understand the biological processes affected by ethylene.
Results and Discussion:
Our transcriptomic analysis revealed significant changes in gene expression patterns associated with ethylene-mediated ripening in A. bisporus. Several key genes involved in ethylene perception, signaling, and downstream responses were differentially expressed during the ripening process. Functional enrichment analysis revealed that ethylene responsive genes were mainly associated with processes such as cell wall modification, fruit ripening, and oxidative stress response. These findings suggest that ethylene plays a crucial role in the regulation of cell wall degradation, flavor development, and physiological changes during post-harvest ripening in A. bisporus.
Furthermore, we identified a set of transcription factors (TFs) that were differentially regulated by ethylene. These TFs may serve as master regulators of the ripening process, controlling the expression of downstream ripening-related genes. Additionally, we found that ethylene-induced oxidative stress may contribute to the initiation and progression of post-harvest ripening in A. bisporus. The activation of antioxidative defense mechanisms was observed, suggesting that ethylene-induced reactive oxygen species (ROS) generation and subsequent ROS scavenging are critical for proper ripening progression.
Conclusion and Future Perspectives:
In summary, our study provides insights into the molecular mechanisms underlying ethylene-mediated post-harvest ripening in A. bisporus. The findings highlight the importance of ethylene perception, signaling, and the involvement of specific transcription factors in regulating the ripening process. Moreover, the activation of antioxidative defense mechanisms suggests a link between ethylene signaling and oxidative stress during post-harvest ripening in A. bisporus. Further research is warranted to validate the role of specific genes and unravel the regulatory network involved in ethylene-mediated ripening. The results obtained from this study can be utilized to develop strategies for optimizing post-harvest storage conditions and prolonging the shelf-life of A. bisporus, thus benefiting the mushroom industry and consumers alike.