毕业论文 初稿.doc

学号:12206010
2010 届本科生毕业论文(设计)
题目:响应面法优化盾叶薯蓣内生菌SYt1发酵条件
学院(系):生命科学学院
专业年级:生物工程061班
学生姓名:杜成志
指导教师:秦宝福副教授
完成日期:
目录
摘要 1
1引言 2
2
2
3
3
内生菌概述 3
内生菌的生物学作用 4
内生菌产生物活性物质研究的意义和展望 5
5
6
7
2 材料与方法 8
8
8
试剂、仪器与设备 8
9
9
9
9
9
9
10
10
3 结果与分析 13
标准曲线的绘制 13
预发酵试验 14
单因子实验 14
Plackett-Burmen 试验 15
16
4 讨论 17
17
-Burman实验的失败 18
18
18
参考文献 19
致谢 22
响应面法优化盾叶薯蓣内生菌SYt1发酵条件
作者:杜成志指导教师:秦宝福
(西北农林科技大学生命科学学院陕西杨凌 712100)
摘要:盾叶薯蓣内生菌SYt1可以合成薯蓣皂苷元。本实验采用单因子实验、Plackett-Burmen 设计、最陡爬坡实验和响应面实验相结合的方法以期对薯蓣内生菌SYt1合成皂素的发酵条件进行优化。通过单因子实验筛选出最适碳源为蔗糖,最适氮源蛋白胨。Plackett-Burmen实验表明蛋白胨、MgSO4和NaCl是影响皂素产量的重要因素。但是最陡爬坡试验结果异常,没有得到最优发酵条件。
关键词:盾叶薯蓣;内生细菌;薯蓣皂苷元;响应面法;优化
Optimization of Fermentation Conditions of Endogenntic Bacterium SYt1 from Dioscroes zingbiberensis using Response Surface Methodology
Author: Du Chengzhi Tutor: Qin Baofu
(College of Life Science,Northwest A&F University,Yangling, Shanxi,712100)
Abstract:Endogenntic bacterium SYt1 isolated from Dioscroes zingbiberensis can produce diosgenin . In this experiment,single-factor bined with Plackett-Burmen design、the path of steepest accent experiment and response surface methodology was conducted to optimize the fermentation conditions to produce diosgenin by SYt1 . By single-factor test the optimum carbon source and nitrogen source confirmed as sucrose and peptone respectively. Plackett-Burmen experiment indicated that peptone、MgSO4 and NaCl were the significant factors that affect the yield of diosgenin. However,the datas of the path of steepest accent experiment were abnormal,and the optimum conditions had not been found.
Keywords: Dioscroes zingiberensis;ic bacterium;