内蒙古大学硕士论文 ANTITUMOR MECHANISMS OF POTASSIUM PIPERATE, A DERIVATIVE OF MONGOLIAN MEDICINE Piper longum L. AB STRACT Piper longum a well-known traditional antihyperlipidemie medicine in China, the constituents extracted from the fruit of P longum yielded piperine,pipemonaline, and ,the antitumor properties of these constituents liFe not understood at found that potassium piperate(GBK),a derivative ofpiperine,has ability to inhibit proliferation of cancer cells,especially mammary cancer also selectively inhibits the G 1-S—phase transition in mammary cancer of G 1 arrest by GBK is correlated with induction of CDK inhibitor p27 expression as well as inhibition of cyclin E,cyclin A and cyclin ’ maintenance(MCM)proteins are markedly in cells treated with the other hand,GBK selectively kills cancer cells by targeting the stress response to might also inhibit cancer cell proliferation through ic signaling ,these results show GBK possesses potential anti. cancer activity against mammary cancer cells by inhibiting cell proliferation and promoting cell apoptosis. KEYWORDS:Mongolian medicine;Piper longum L.;Antitumor mechanisms II 万方数据 内蒙古大学硕士论文 目录 第一章:研究背景概述(文献综述) l 1 l: 1 1 .2 一5 5 Caspase家族 一6 Caspase激活机制 ..9 9 ..10 .11 第二章蒙药荜茇有效成分衍生物胡椒酸钾抗肿瘤机理 13 1 3 .15 .15 ..1 6 .17 ..2l 22 GBK对正常细胞和癌细胞毒性测定 .22 。2 GBK作用后细胞活性测定 .22 GBK药物的选择性抑制作用实验 .23 GBK与依托泊苷联合用药对细胞活性的的影响 .23 万方数据 内蒙古大学硕士论文 .:23 GBK对肿瘤细胞周期的影响 .24 .25 RNA反转录实验 .26 PCR .26 2.:;.10 Western Blot .27 GBK对细胞凋亡的影响 29 Caspase 3及Caspase
