谷胱甘肽及谷胱甘肽与金属离子体系的电化学行为分析及其应用.docx

摘 要谷胱什肽是一种在生物体内广泛存在的生命物质,它在生物体内
具有重要的生理功能,而且在医药、食品等行业具有很大的应用价值, 此外,谷胱IjJ肽也是研究生物体内蛋白质与金属离子相互作用的很好的模型化合物。因此,对谷胱_|:j-月太及谷胱甘肽与金属离子体系的电化
学行为进行研究,有助于从分子及分子集合体水平上进一步了解谷胱茸一肽在生物体内的生理功能及生物体内谷胱甘肽与金属离子的相互作Hj机制。
本文采用循环伏安法、电毛细管曲线等电化学研究方法研究了
()、()、()()中的电化学行为,对谷胱甘肽在汞电极上的电化学反应机理进行了探讨,并提出了较为详细的电极反应机理;同样利用这些电化学研究方法研究丁谷胱甘肽与铜(II)、镉(II)()中的电化学行为,对两个体系在汞电极上的电化学反应机理进行了探讨,提出了较为详细的电化学反应机理,并分别利犀}谷胱甘肽一铜(II)、(II) 离予体系在JP-4000型及JP一2型极谱仪上对谷胱甘肽进行了检测, 并应用于阴药(依士安滴眼液)中谷胱甘肽含量的测定,结果满意。
关键词:芥胱甘肽,铜,镉,循环伏安法,电毛细管曲线
Abstract
The tripeptide glutathione(GSH)is a kind of pound existing extensively anism,it has essential physiological function anism,and in medicine,fi)od industry it has also very important using addition,it's a very good model pound of smdying the reaction mechanism of protein and metal ions interaction in the ,in this paper,the electrochemical behavior of GSH,GSH
—metal ions systems was studied.
The electrochemical behavior of GSH in ,C00H.
C1Ct乇COONa(),O,2mol/LHAc·NaAc(),-IP04 一NaH2P04(),,H20—NH4C1()buffer solutions was studied with Cyclic Voltm-nmetry and electrocapillary curve experiments and paratively detailed electrochemical reaction mechanisln was proposed.
The electrochemical behavior of GSH—Cu2+ion + ion system in phosphate buffer(、was also studied with Cyclic ry and electrocapillary curve experiments,and paratively detailed electrochemical reaction mechanism was results were utilized to establish the quantitative methods of GSH determination with linear sweep conditions optimization,we determined the content of GSH in medicine and the
results are satisfactory.
Keywords:glutathione,coppeg cadmium,Cyclic Voltammetry,
electrocapillary Curve
械川坪:谷胱陆^乏谷胱¨肽马盒属离子体系的f眭化学行为研究艇其I坦一一—二上
引 言
当4-E(,I学的-个曼薯特点裁是化学磅究越来