山西医科大学硕士学位论文 中药双利肝的质量标准实验研究 摘 要 1、丹参、大黄混合液及其丹参中丹参酮IIA、丹参素的含量测定目的:对丹参及丹参与大黄混合提取液(双利肝)中的有效成份进行了 定量测定并且比较了混合前后含量的变化,同时考察了不同提取方法对丹参 酮IIA、丹参素的影响。 方法:采用RP—HPLC(高效液相色谱法)测定丹参素、丹参酮IIA的 含量。以十八烷基硅烷键合硅胶为填充剂;%醋酸 (13:87)为流动相,检测波长280nm丹参酮IIh以甲醇一水(78:22)为 流动相,检测波长270nm;按外标法定量。结果与结论:方法准确度,回收率均符合中国药典II部(2000版)规定。 灵敏度高、专属性强。 2、大黄、丹参混合液及其大黄中大黄素的含量测定 目的:对大黄及丹参与大黄混合提取液(双利肝)中的大黄素进行了定量测定并且比较了混合前后含量的变化,同时考察了不同提取方法对大黄素含量的影响。 方法:采用RP_埘PLc(高效液相色谱法)测定大黄素的含量。以十八烷基硅烷键合硅胶为填充剂;以甲醇—%磷酸(90:10)为流动相,检测波长 254nm;按外标法定量。 结果与结论:方法准确度、回收率均符合中国药典II部(2000版)。灵敏度高、专属性强。 山西医科大学硕士学位论文 关键词:双利肝 丹参酮IIA 丹参素 大黄素 反向高效液相色谱q水—HP味~ 含量测定 .2. 1 t ; ;。} ,j ㈡疆添、麓。魏。麓黼;.; 山西医科大学硕士学位论文 Experimental Studies of Shuangl igan on Qual i ty Standard Abstract 1 Methods for the quantitative determination of the active constituent in Danshen Dahuang mixture and Danshen crude drug Objective:To establish methods for the quantitative determination of Tanshinone II A and Danshensu in Danshen Dahuang mixture and Danshen crude drug,investigate the quantitative variety among Danshen Dabuang the effects of several extraction methods pared in the quantitative determination of the active constituents. Methods:Reverse phase high performance l iquid chromatography (RP-HPLC)was 250砌× TIANHE-oDS column with a mobile phase consisting of %ethyl acid(13:87)was used for was detected at 250 mmX TIANHE—0DS column with a mobile phase consisting of methanol—H20(78:22)was used for was detected at 270册. Result and conclusion: Both of the methods had good repeatability,sensitivity and specificity. 2 Methods for the quantitative determination of the active constituent in Danshen Dahuang mixture and Dahuang crude drug Objective:To establish the method for the quantitative determination of Dahungsu in Danshen Dahuang mixture and Dahuang crude drug,investigate the quantitative variety among Danshen Dahuang .3. }|;巍+ 罐巍垂囊鼓_ ∥羹囊{ 山西医科大
