注射用匹多莫德的免疫活性分析.docx

Experimental Studies on Immunological Activity of Pidotimod for Injection
Candidate: Chen lijue Supervisor: Asso. Prof. Zeng Fanbo
Abstract
Pidotimod is a synthetic substance with important immunomodulatory properties both in vitro and in vivo. Pidotimod is able to activate natural killer cell, enhance the phagocytic function of macrophage and neutrophilic granulocyte, promote the proliferation of lymphocyte induced by mitogens, and increase the production of cytokines such as Interleukin 2 and γ-interferon. Pidotimod is often utilized together with antibiotics and antivirotics to cure infectious diseases. Pidotimod is mostly used as a preparationsfororaluse, however, under some circumstances, it is necessary to make pidotimod into injection in order to speed up the absorption and increase its bioavailability. In this reaseach, the immunological activity of Pidotimod for injection has been studied.
In chapter one, the effect of Pidotimod for injection on humoral immunity function and ans index of Balb/c mice was studied. In this experiment, 60 Balb/c mice were used, 36 of which were injected into Pidotimod with different doeses, 12 of which were injected into normal saline as negative control, and the rest of the mice were intragastrically perfused with Levamisole as positive control. All of the mice were doesd one time per day and were dosed for 14 times altogether. After 10 days of treatment, half
of the mice were intraperitoneally injected with cyclophosphamide 80 mg·kg-1 one time
per day for 4 times to establish the immunosuppressive model. On the 10th day of the treatment, all of the mice were intraperitoneally injected with SRBC. After the treatment, the serum hemolysin formations were performed by HC50 assay; Weights of thymuses and
spleens of mice were measured. Result:The HC50 of each dosage of Pidotimod on normal
mice was ±, ±, ±, respectively, there was no significant difference between each dosage of Pidotimod