透明质酸刺激因子对增生性瘢痕成纤维细胞透明质酸合成的影响.doc

透明质酸刺激因子对增生性瘢痕成纤维细胞透明质酸合成的影响
作者:杨力鲁开化王强艾玉峰李荟元王胜春
【关键词】,刺激剂
关键词: 刺激剂;成纤维细胞;透明质酸;瘢痕,肥大性
摘要:目的了解兔源性透明质酸刺激因子(HASF)对体外单层及三维培养条件下增生性瘢痕成纤维细胞(FB)透明质酸(HA)合成的影响. 方法从胎兔羊水及血清中提纯HASF分别作用于单层及三维培养的FB细胞,用放免法测定上清液中HA浓度. 结果在单层培养条件下,・L-1 ・L-1 ,HA浓度也由(495±37)mg・L-1 增至(914±75)mg・L-1 ,在一定范围内呈剂量依赖性(P<).继续增加HASF剂量,HA浓度并不成比例增加,,HA浓度也呈时间依赖性的增加(P<).在三维培养条件下,随HASF剂量的增加,HA浓度也由(90±25)mg・L-1 增至(293±63)mg・L-1 ,也呈剂量依赖性(P<),且明显低于单层培养条件下HA的浓度(P<). 结论 HASF对单层及三维培养的FB有明显的促HA合成作用,并呈剂量和时间依赖性.

Keywords:irritants;fibroblasts;hyaluronic acid;cicatrix,hypertrophic

Abstract:AIM To investigate the effect of hyaluronic acid stimulating factor(HASF)on the hyaluronic acid(HA)syn-thesis of hypertrophic scar-derived fibroblasts cultured three-dimensionally in the HASF,isolat-ed from the fetal rabbit sera and amniotic fluid,was added to the monolayer and three-dimensional fibroblast culture sys- concentration of HA in the supernatant of the fi-broblast culture systems was measured using radioim┐ Concentration of HA in the mono-layer system increased from(495±37)mg・L-1 to(914±75)mg・L-1 in a dose-dependent manner with the increase of HASF ・L-1 ・L-1 (P<).The concentration of HA reached the maximam when HASF a