07 Application of Bacteria In Wastewater Treatment ChaiYS.pdf

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THE BACTERIA

Composition  C60H87O23N12P

Size
-6 μm wide & 2-20 μm long

Shape / morphology
High power microscope (x1000)

Staining techniques

Criteria:

pH
pH optimum: pH 7

Temperature
Thermophiles: 35-75º C

Mesophiles: 20-50º C

Psychrophiles: 10-30º C

Oxygen requirements  Aerobic
 Anaerobic
 Facultative
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BIOLOGICAL TREATMENT
CLASSIFICATION
Suspended Growth Activated Sludge
Method System
Aerobic
Treatment
Attached Growth Trickling Filter
Method
Anaerobic Anaerobic Digestion (POME)
Treatment
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Anaerobic
Digestion
(POME)(POME)(POME)
OverviewOverviewOverview
Fresh POME is a thick brownish colloidal slurry of
water, oil and fine suspended solids with an
obnoxious odour.
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ProProcesscess Microbiology
In an anaerobic process, a consortium of
anisms catalysing plex series of
biochemical reactions that anic
matter producing methane and CO2
Organic Matter
Anaerobic Other
+++ +++ Energy +++ Methane +++ COCOCO +++
MicrobesMicrobesMicrobes 222 gases
Combined Oxygen
-2 -2 -1 -3
** Combined Oxygens: CO3 , SO4 , NO3 , and PO4
** Biogas ~ 60% methane & ~ 40% carbon dioxide, with trace amounts of
nitrogen, hydrogen, and hydrogen sulfide
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ANIC MOLECULES
Carbohydrates
Proteins Lipids
HYDROLYSIS
anic Molecules
Amino Acids Sugars Fatty Acids
NH
ACIDOGENESIS 3
Volatile Fatty Acids
H2S
Propionate, Butyrate, etc
H
ACETOGENESIS 2
Acetic Acid
H2 COCOCO22 22
METHANOGENESIS
Methane CO2
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HYDROLYSIS

Hydrolytic Bacteria
 Facultative or strict anaerobes.

Responsible for 1st stage:
 complex molecules  simple monomers

Produced a number of extracellular hy