Ultrastructure, scanning electron microscopy of anastomosis site were observed after 1、3、7、14 days postoperation. 3.
On the basis of the second part, the serum of the TNF-αconcentrion were tested preoperation and analysis was utilized to observe the microstructure at the anastomosis site. Results 1.
Typical diabetic symptoms and
signs appeared in STZ-induced Diabetic rats. Obvious statistical diference was observed between the two groups. Morphological investigation on pancreas after 8 weeks sympotoms showed the reduction of islets,loss of β cells in the Diabetes group. 2.
Subendothelial basement membrane became thicker and local atherosclerosis could be observed in the Diabetes rat’s carotid artery.
Smooth muscle cells migrated into the intimal cells,where they underwent phenotypic transformation from contractile to secretory carotid structural remodeling in the Diabetes rats expressed as vascular hypertrophy. After 8 weeks sympotoms in the Diabetes groups,the carotid artery’s outer diameter, thickness and area was increased;ponents of carotid artery expressed as the increase of collagen and the decrease of elastin. Prior to collagen increase and elastin dreased,which resulted in the C/E ratio increased by degrees. 3.
The intimal cells injury at the microarterial anastomosis site were conspicuous in the Diabetes pared with the control groups,while obvious inflammation and granulation tissue hyperplasia around the anastomosis site in the Diabetes groups was 7 days postoperation,the average area of exposed suture surface at microarterial anastomosis site was ± m2 in the Diabetes group compared to ± in the normal group (p<). The degree of intimal repair was significantly lower in the Diabetes pared to the control group. At 14 days postoperation,intima/media ratio at the anastomosis site was markedly increased in the Diabetes group than the co
