吡喹酮透皮给药治疗小鼠日本血吸虫病.pdf

上海市畜牧兽医学会 2011 年学术年会论文集

吡喹酮透皮给药治疗小鼠日本血吸虫病
李小红,杨艺,江艳,刘毅,徐玉梅,朱传刚
(中国农业科学院上海兽医研究所,上海 200241)
摘要:目的:本文旨在探究吡喹酮(Praziquantel,PZQ)透皮剂不同透皮剂量和次数的治疗效果,以及吡
喹酮透皮剂对不同发育阶段和再感染早期日本血吸虫的治疗效果,考察吡喹酮与宿主免疫系统是否具有协
同作用,并为日本血吸虫病提供更加便利的治疗途径。方法:各试验组昆明小鼠均感染日本血吸虫尾蚴 40±2
条,感染后 35 d 采用不同透皮剂量的吡喹酮透皮剂和不同透皮次数治疗昆明小鼠,并对一次感染后不同发
育阶段和再次感染早期的昆明小鼠进行透皮治疗。各试验组均设对照组,感染后 42d 解剖冲虫,计算减虫
率、减肝卵率和减毛蚴孵化率,评价透皮剂的治疗效果。ELISA 检测初次感染和再次感染不同时期 IgG 抗
体水平的动态变化。结果:感染日本血吸虫 35d 后吡喹酮透皮剂透皮高剂量减虫率为 %,低剂量透皮
3 次减虫率为 %,效果较好;对不同发育阶段日本血吸虫的治疗试验中,童虫期减虫率在 20%~50%之
间,对 21 d、28 d 成虫减虫率在 70%以上;感染后 1 d 和 14 d 的透皮治疗,感染二次的减虫率都在 80%以
上,比初次感染后 1 d 和 14 d 的透皮治疗效果好。结论:PZQ 透皮制剂杀灭血吸虫的效果具有剂量和次数
依赖性,少量多次治疗效果较好;透皮剂对不同发育阶段的日本血吸虫均有不同程度的杀灭作用,对童虫
有一定的杀灭作用,但对 21 d、28 d 成虫杀灭作用更显著;对再感染早期童虫的减虫效果更好,说明吡喹
酮可能与宿主具有免疫协同性。
关键词:日本血吸虫;吡喹酮;透皮治疗
TRANSDERMAL DELIVERY OF PRAZIQUANTEL FOR THE
TREATMENT OF THE SCHISTOSOMIASIS OF MICE
Abstract:Objective:This article aims to explore the effective of transdermal agent of PZQ by different dose and
treat times, and the effective of the transdermal agent of praziquantel on different developmental stages and the
early period of re-infection of Schistosoma japonicum: whether praziquantel and host immune system had
synergistic effect or not,and provided more convenient access to treatment of schistosomiasis. Methods:All of the
experiment groups of Kunming mice were infected with Schistosoma japonicum cercariae by 40 ± 2, the Kunming
mice were infected by Schistosoma japonicum after 35 d , they were treated through abdominal transdermal with
different dose and treat times;the Kunming mice which was infected once at different developmental stages and the
early period of re-infection of Schistosoma japonicum were treated by abdominal all of the
experiment groups set control groups, all of the mices were dissected 42 d after infection,it calculated worm
reduction rate, egg reduction rate and miracidia hatching reduction rate of treatment groups,it evaluated the