脂肪干细胞介导的自体脂肪移植隆胸.docx

中国组织工程研究  2013, Vol. 17  Issue (5): 878-885    
DOI: .2095-
组织移植
脂肪干细胞介导的自体脂肪移植隆胸
Auto-adipose stem cells facilitate autologous fat transfer for breast augmentation
全文: PDF (743 KB)
摘要 
背景:脂肪干细胞具有多向分化潜能,在组织工程和再生医学领域具有广阔的应用前景。
目的:随访评估脂肪干细胞在自体脂肪移植隆胸中的技术要点及治疗效果。
方法:随访15例自体脂肪颗粒注射隆胸患者3-6个月。脂肪移植隆胸过程分为两期,一期用于脂肪干细胞的分离与扩增,抽出脂肪量为10-20 mL。用胶原酶Ⅰ消化,贴壁培养获得脂肪干细胞,扩增至第2代达到1×108,并进行细胞免疫表型,分化及遗传稳定性鉴定。二期抽取脂肪量300-450 mL,脂肪混合脂肪干细胞用于移植填充。
结果与结论:第2代自体脂肪干细胞具有典型的间充质干细胞的特征,移植后3-6个月回访无明显脂肪吸收现象,无感染、疼痛、上肢活动受限等不良反应,隆胸效果良好。作者认为自体脂肪干细胞介导的自体脂肪注射隆胸是一种切实可行有效的方法。
关键词 : 器官移植; 组织移植; 组织细胞移植; 脂肪干细胞; 间充质干细胞; 遗传稳定性; 免疫表型; 分化潜能; 隆胸; 自体脂肪颗粒注射; 器官移植细胞图片文章;   
Abstract:
BACKGROUND: Human adipose stem cells have the capability of multipotential differentiation and have a promising prospect in the field of tissue engineering and regenerative medicine.
OBJECTIVE: To evaluate the technology and efficacy of human adipose stem cells in autologous fat grafting breast augmentation.
METHODS: Fifteen patients with auto-adipose injection breast augmentation were followed-up for 3-6 months. The whole operation was separated into two phases, in the first phase, 15-20 mL adipose tissue was aspirated and digested with collagenase Ⅰ to separate and amplify the human adipose stem cells. When cultured to passage 2, the plastic adherent stem cells were up to 1×108 and verificated by immunophenotype