吉西他滨增强肝癌HepG2 细胞体外辐射敏感性的机制研究.pdf

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2011;31(12) 南方医科大学学报(J South Med Univ) 1993
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基础研究
吉西他滨增强肝癌HepG2细胞体外辐射敏感性的机制研究
凌志海,孙权权,张耀伟,官键,丁轶,陈龙华
南方医科大学南方医院放疗科,广东广州 510515
摘要:目的探讨吉西他滨对肝癌HepG2细胞辐射增敏作用的影响及机制的初步研究。方法采用克隆形成实验计算不同
剂量照射后L-Q模型放射生物学参数比和放射增敏比;应用流式细胞技术检测Gemcitabine(GEM)和辐射前后细胞周期
及凋亡率的变化。结果经GEM处理后的HepG2细胞组,Survival fraction at 2 Gy (SF2)较未处理组显著偏低,α值显著增
高,GEM处理后增加了辐射诱导的G0/G1期细胞阻滞和细胞凋亡。结论 GEM可显著增强HepG2细胞的辐射敏感性,这
种作用是通过增强辐射诱导的G0/G1期细胞阻滞和细胞凋亡来实现的。
关键词:吉西他滨;肝癌;辐射
中图分类号:R81 文献标志码:A 文章编号:1673-4254(2011)12-1993-04
Effect of gemcitabine in enhancing the radiosensitivity of HepG2 hepatoma cells and the
possible mechanism
LING Zhi-hai, SUN Quan-quan, ZHANG Yao-wei, GUAN Jian, DING Yi, CHEN Long-hua
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Abstract: Objective To evaluate the effect of gemcitabine in enhancing the radiosensitivity of hepatoma cell line
HepG2 and explore its mechanisms. Methods Clonogenic survival assay is employed to calculate the ratios of L-Q
model radiation biology parameters and radiosensitization after different doses of irradiation. Flow cytometry was
used to detect the changes in HepG2 cell cycle and apoptosis rate after gemcitabine treatment and radiation exposure.
Results The survival fraction at 2 Gy of HepG2 cells treated with gemcitabine was significantly lower, and the value of
α was si