HPLC法测定芦丁中芦丁和槲皮素的含量.doc

HPLC法测定芦丁中芦丁和槲皮素的含量
李启彬1,刘涛2,曹晓庆,李青丽(,驻马店463000;
,驻马店463000)
摘要:目的利用HPLC测定芦丁中芦丁和槲皮素的含量。方法采用Zorbox SBC18色谱柱,流动相为水-甲醇-乙酸(45:50 :5), · min-1 ,检测波长256nm。结果芦丁在60 ~ 140μg · ml-1 浓度范围内,线性关系良好,r = 000, ~ · ml-1 浓度范围内, 线性关系良好,r=。%,%,%,%。结论本法结果准确,重复性良较好,方法简便可行。
关键词:芦丁;槲皮素;HPLC
Determinaition of Rutin and Its Quermination by HPLC
LI Qi-bin1,LIU Tao2, CAO Xiao- qing,LI Qing-li(1. Zhumadian Institute of Quality Control of Drug, Zhumadian 463000,China;2. Henan Topfond Pharmaceutical Co.,Ltd Zhumadian463000,China)
ABSTRACT: OBJECTIVE A HPLC method for the determination of rutin and its quermination was established. METHODS A zorbox SBC18 column was used,with the mobile phase of water-methanol-Vinegar(45:50:5), at the flow rate of · min-1,the detection wavelength was 256nm. RESULTS The linear range of rutin (r=) was 60~140ug·ml-1,and quermination (r=) was ~ · ml-1 ,The average recovery of Rutin was %,%,and quermination was %,%.CONCLUSION It was accurate and rapid.
KEY WORDS: rutin; quermination; HPLC
芦丁又名芸香苷、芸香苷是从槐米中提取的一种浅黄色或微绿色针状结晶或粉末。它具有降低毛细血管的异常通透性和脆性的作用。主要用于防治高血压病的辅助治疗。同时,它又是合成曲克芦丁的主要原料。现行卫生部药品标准采用紫外分光光度法测芦丁含量,用纸层析法测槲皮素的含量,方法复杂,操作费事,并且槲皮素无法定量,而用HPLC法操作简便,结果准确。
1 仪器和试药
仪器
高效液相色谱仪: Agilent 1100系列二级管阵列检测器,HP ChemStation化学工作站,Ag