人参皂苷Rg1、Rb1、Rd对大肠杆菌生物热活性的影响.doc

人参皂苷Rg1、Rb1、Rd对大肠杆菌生物热活性的影响
曹俊岭1,李宏1,范秀荣1,肖小河2*国家自然科学基金项目(30500670);国家重点基础研究发展计划(973计划):2007CB512607
,付鹏1
1 北京中医药大学东直门医院药学部北京100700
2 解放军302医院全军中药研究所北京100063
摘要目的:探讨人参皂苷Rg1、Rb1、Rd对大肠杆菌生物热活性的差异,为中药的活性筛选构建新的方法。方法:应用生物热动力学的方法,对人参皂苷Rg1、Rb1和Rd对生物模型的热活性的影响进行研究,计算总体发热量(Q),最高发热量(Pmax),最高发热时间(Tmax)代谢速率常数1(k1)和代谢速率常数2(k2)等热力学参数,应用主成分分析等统计方法对数据进行处理,并应用琼脂扩散法对各种皂苷的抑菌活性进行验证。结果人参皂苷Rg1、Rb1在小剂量时对大肠杆菌代谢产热有轻微的促进作用,但随着剂量的增加则作用下降且产生抑制作用,但二者没有明显的差别;人参皂苷Rd从小剂量时就可使Pmax降低,k2下降,并随着剂量的增加而增加(P<),且在同等剂量下,其降低Pmax、k2的作用明显强于人参皂苷Rg1、Rb1(P<),各种皂苷的常规抑菌活性与生物热活性结果基本一致。结论人参皂苷Rd对大肠杆菌的代谢产热有较强的抑制作用,人参皂苷Rg1、Rb1次之;同时为生物热动力学方法在药效物质筛选方面的应用提供依据。
关键词人参皂苷Rg1、Rb1、Rd 大肠杆菌生物热活性
Effects of Biothermo-activity of 3 Genosides on Escherichia coli
Cao Juning, Li Zulun, Xiao Xiaohe 1 Pharmacy Department, Dongzhimen Hospital Affiliate to Beijing University of TCM (Beijing 100700); 2 PLA Institute of Chinese Material Medica,302 Hospital of PLA (Beijing 100039 ); 3 College of Pharmacy, Chengdu University of TCM (Sichuan 610071)
Abstract objective:To investigate the difference of biothermo-activity of 3 genosides on Escherichia coli and establish the method for activity screening of Chinese material medica. Method: ics was used to examine the effects of different genosides on Escherichia coli. Thermodynamics parameter, such as Q , Pmax, Tmax, k1 and k2 were calculated. And they were analysis by ponent analysis. Result: Ge