CD59结合短肽对补体介导的前列腺癌细胞溶解影响.doc

CD59结合短肽对补体介导的前列腺癌细胞溶解影响
【摘要】目的观察CD59结合短肽(sp22)对补体介导高表达人CD59分子的PC3细胞溶解的影响。方法利用脂质体法将携带人野生型CD59基因的pIRES质粒(wCD59pIRES)转染PC3细胞,G418筛选稳定转染的细胞株,荧光免疫组化技术检测细胞表面CD59分子的表达,补体溶解试验观察sp22和抗CD59单克隆抗体对补体介导的高表达CD59分子的PC3细胞溶解的影响。结果高表达CD59分子的PC3细胞株构建成功;与对照组比较,sp22组转染细胞的溶解率显著升高(F=,q=~,P<);相同浓度条件下,sp22组转染细胞的溶解率明显高于抗CD59单克隆抗体组(q=~,P<)。结论 sp22可封闭PC3细胞表面高表达CD59分子的补体结合位点,显著增强抗PC3细胞抗体所诱发的补体介导的抗瘤作用。
【关键词】抗原,CD59;短肽;前列腺肿瘤;补体溶解试验
[ABSTRACT] Objective To investigate the effect of short peptide binding to CD59 molecules on lysis plementmediated prostate cancer PC3 cells which highly expressed transfected human wildtype CD59 gene. Methods pIRES vectors carrying wildtype CD59 gene (wCD59pIRES) were transfected into PC3 cells by the lipofectin. Transfected PC3 cells were screened by the addition of G418. The expression of CD59 on PC3 cells was detected by fluorescence immunohistochemistry technique. The effect of sp22 or anti
CD59 monoclonal antibody plementmediated cytotoxicity to transfected PC3 cells was evaluated plement lysis assays. Results PC3 cells highly expressing CD59 molecules were essfully constructed. Compared with the control group, the lysis rate of transfected PC3 cells in sp22 group was increased significantly (F=,q=-,P<). At the same concentration of sp22 and antiCD59 monoclonal antibody, the lys