妊娠滋养细胞疾病组织中端粒酶逆转录酶表达的研究.doc

妊娠滋养细胞疾病组织中端粒酶逆转录酶表达的研究
作者:周健,毕淑宁,任玲,薛华,林萍,李桂林

【关键词】妊娠滋养细胞疾病
摘要:目的探讨人端粒酶逆转录酶(hTERT)在妊娠滋养细胞疾病中的表达及其临床意义。方法采用兔抗人端粒酶逆转录酶抗体,利用免疫组织化学方法检测29例妊娠滋养细胞肿瘤、18例葡萄胎和20例正常早孕绒毛组织中hTERT的表达,结合Leica Qwin图像分析系统对hTERT阳性细胞进行灰度分析。结果 hTERT表达定位于细胞质。hTERT阳性细胞平均灰度值在妊娠滋养细胞肿瘤、±、±±。hTERT在妊娠滋养细胞肿瘤中的表达明显高于葡萄胎和早孕绒毛组织,差异有显著性(P<)。结论端粒酶的活化在妊娠滋养细胞肿瘤形成中可能起重要作用,端粒酶活性的检测可能是预测葡萄胎预后的重要指标。

关键词:妊娠滋养细胞疾病;人端粒酶逆转录酶;免疫组织化学

Abstract:Objective To investigate the expression of human telomerase reverse transcriptase(hTERT)in gestational tro-phoblastic diseases and its clinical In29gestational tropholbastic tumors(GTT),18hydatidiform moles and20normal early embryonic villi,the
expression of hTERT was studied by using immunocytochemical staining and image analysis The hTERTwas localized in cytoplasm,and its average grey level ±,±± GTT,hydatidiform moles and early embryonic villi expression of hTERT was significantly higher in GTTthan in hydatidiform moles and early embryonic villi(P<),and showed no difference between those in hydatidiform moles and early embryonic villi(P>).Conclusion Telomerase activation may play an important role in the genesis of trophoblastic of telomerase activity is of potential value in making the prognosis