人参皂苷Rb3对大鼠局灶性脑缺血损伤的保护作用.doc

人参皂苷Rb3对大鼠局灶性脑缺血损伤的保护作用
作者:胡瑜,陈浩凡,臧林泉,巫玮
【摘要】目的探讨3种人参皂苷Rb3对大鼠局灶性脑缺血损伤的保护作用。方法将48只大鼠随机分成6组:假手术组、模型组、人参皂苷Rb3 20、40、80 mg/kg组和阳性对照药血栓通注射液(XST)组;采用插线法使大脑中动脉闭塞(MCAO)建立大鼠局灶性脑缺血模型,于造模24 h后进行行为学评分,测定脑组织含水量以及脑组织中超氧化物歧化酶(SOD)和丙二醛(MDA)含量,计算脑梗死面积百分比,并进行组织病理学检查。结果人参皂苷Rb3 40、80 mg/kg能明显缓解脑缺血大鼠的行为学症状,减轻其脑水肿程度,降低血清中MDA含量,升高SOD含量,并能减少其脑梗死面积,减轻组织病理学损伤。结论人参皂苷Rb3对大鼠局灶性脑缺血损伤有较明显的保护作用。
【关键词】脑缺血; 大脑中动脉闭塞(MCAO);人参皂苷Rb3
Protective effects of ginsenoside Rb3 on focal cerebral ischemic injury in rats
Abstract:Objective To study the protective effects of ginsengoside Rb3 on focal cerebral ischemia in 48 SD rats were divided randomly into sham
operated group, model group, ginsenoside Rb3 (20, 40 and 80 mg/kg) group and positive control drug (XST) group. The model of focal cerebral ischemia was made by middle cerebral artery occlusion (MCAO) with nylon suture; neuroethological scores were gotten 24 h after the development of the model. The water content of brain tissue, the activities of SOD and MDA in brain tissue and were measured, the percentage of cerebral infarction area to total brain area was calculated, and histopathological examination was conducted. Results Ginsenoside Rb3 (at 40 mg/kg and 80 mg/kg) could significantly reduce the scores for neurological deficits and brain edema, improve the activities of SOD, lower the content of MDA, reduce the cerebral infraction area, and alleviate the cerebral ischemia injury. Conclusion Ginsenoside Rb3 had obvious protective effects on the focal cerebral ischemia in rats.
Key words:brain ischemia;middle cerebral artery occlusion (MCAO);ginsenoside Rb3
脑卒中是造成人类致死和致残三大原因之一,脑对缺血缺氧极为敏感,脑缺血会引起脑组织不同程度的损伤,严重者影响中枢神经系统的功能。目前对缺血性脑卒中神经元损伤的机理进行了深入的研究[1],认为自由基的生成增加、中性粒细胞及巨嗜细胞的聚集、蛋白溶解酶的释放以及再灌注后血脑屏障的双相性开放是脑缺血再灌注损伤的发病机制。另外血脑屏障完整性在脑缺血后再灌注中发挥重要的病理生理作用:脑缺血再灌注后可使脑梗死及血脑屏障的破坏加重,且血脑屏障的破坏与脑血流的恢复存在相关性,局灶性脑缺血早期再灌注虽然有助于保护半暗区组织,但迟发性再灌注可加重脑损伤。脑梗死早期予以溶栓恢复缺血区脑血流可以减轻缺血性损伤,然而延迟再通可增加出血转化及脑水肿的危险[2],这限制了溶栓治疗的应用与推广。因此开发防治脑组织缺血性损伤的药物,延长血流再通的有效时间窗是有待解决的问题,也