Programmed cell death-1 is expressed in large retinal ganglion cells and is upregulated after optic nerve crush》.pdf.pdf

Programmed cell death-1 is expressed in large retinal ganglion cellsand is upregulated after optic nerve crushWei Wanga,b, Ann Chana, Yu Qina, Jacky . Kwonga, Joseph Capriolia,Ralph Levinsona, Ling Chena,b,**, Lynn K. Gordona,*Jules Stein Eye Institute, David Geffen School of Medicine at the University of California, Los Angeles, CA 90095, United StatesFudan Af?liated Eye and ENT Hospital, Fenyang Road 83#, Shanghai 200031, Chinaarticle infoArticle history:Received 17 September 2014Received in revised form14 July epted in revised form 3 August 2015Available online 13 August 2015Keywords:Programmed cell death-1Large retinal ganglion cellsApoptosisOptic nerve crushNeuroprotectionabstractProgrammed cell death-1 (PD-1) is a key negative receptor inducibly expressed on T cells, B cells anddendritic cells. It was discovered on T cells undergoing classical programmed cell death. Studies showedthat PD-1 ligation promotes retinal ganglion cell (RGC) death during retinal development. The purpose ofthis present study is to characterize PD-1 regulation in the retina after optic nerve crush (ONC). C57BL/6mice were subjected to ONC and RGC loss was monitored by immunolabelling with RNA-binding proteinwith multiple splicing (Rbpms). Time course of PD-1 mRNA expression was determined by real-time -1 expression was detected with anti-PD-1 antibody on whole mount retinas. PD-1 sta