趋化因子mip-1α和mcp-r-r-..doc

趋化因子MIP-1α和MCP-R-R-2在慢性髓系白血病细胞中的表达(1)    】本文旨在研究MIP-1α和MCP-R-R-2在慢性髓系白血病细胞中的表达,同时观察P210bcr/abl融合蛋白中酪氨酸激酶对MIP-1α和MCP-R-R-2mRNA表达的影响。在bcr/abl融合基因阴性和阳性慢性髓系白血病细胞中,采用半定量RT-PCR方法检测MIP-1α、MCP-R-R-2mRNA的表达水平。结果表明MIP-R-1mRNA在bcr/abl融合基因阳性细胞中不表达,但在bcr/abl融合基因阴性细胞中表达,而MCP-R-2mRNA在bcr/abl融合基因阳性和阴性细胞中均不表达。当P210bcr/abl融合蛋白中酪氨酸激酶被抑制后,MIP-R-1的mRNA表达水平恢复至正常水平。结论:P210bcr/abl融合蛋白抑制慢性髓系白血病细胞中MIP-R-1mRNA的表达,但对MCP-R-2mRNA的表达无影响。【关键词】慢性髓系白血病 ExpressionsofMIP-1α,MCP-R-R-2inChronicMyeloidLeukemiaCells AbstractThisstudywasaimedtoexploretheexpressionofMIP-1α,MCP-R-R-2inbcr/ablfusiongenepositiveCMLcells,andtostudytheeffectsofP210bcr/ablfusionproteintyrosinekinaseonexpressionofMIP-1α,MCP-R-R--1α,MCP-R-R-2mRNAweredetectedbysemi-quantitativeRT-PCRinbcr/ablnegativecells,bcr/ablpositivecells,andP210bcr/abl-Rb-C--R-1mRNAswereexpressedinbcr/ablnegativecells,-R-2mRNAcannotbedetectedinbothbcr/-C-Box,expressionsofMIP-R-1mRNAswererestoredtonormal(similartoP210bcr/ablnegativecells).ItisconcludedthatP210bcr/ablfusionproteininhibitstheexpressionofMIP-R-1inchronicmyeloidleukemiacells,butdoesnotinhibitMCP-R-2mRNAexpressionsintheseleukemiacells. Keywordschronicmyeloidleukemia;bcr/ablfusiongene;P210bcr/ablfusionprotei