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癌胚抗原和癌抗原15-3电化学免疫传感器地研究.pdf

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patibility、ⅣiththeenVirO咖entforavoidingthepmteinsdenaturalization,}lemainworksandconcIusionsareincIudedasfbllows:-ampIi疗cationstrategyofelectrochemicalsi印aling舶mant培朗。柚tibodyinteractionswasproposedviabacb行1linggoldnanoparticleson(3一mercaptopropyl)订imethoxysilanesoI-gcl(MPTS),suchaswitllnanogoldbutnotⅣIPTSorwithⅣ【PTSbutnotnallogold,wereinvestigatedforthedeterm{nationofcarcinoembryonicantigen(CEA)髂amodel,,thefomlationoftheantigen-plexbyasimpIeone—stepimmunoreactionbet、veentheimmobilizeda11ti-CEAaIldCEAinsaIllplesolutionintroducedmembranepOtentialchangebeforeandaftert11e绷tigen-,oncentrat{·mL~·mL‘1(at36).Moreover,theprecision,reproducibilitya11dstab订ityoft}1eas-preparcdimmunosensor、ve∞,—ninationofcarcinoembryOnic柚tigen(CEA)basedonDNA,nano-,whichcontaillsDNA(negativelycharged)a11dnaIlo-Ag,cannot0nlybeimmobilizedtothegl嬲scarbonelectrodcsurf缸emodmedw油poly·thione(positivelycharged)throughnegativeIychargedDNA,-,—·mL.‘。mL~.

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