柑橘黄酮中总黄酮及橙皮苷的含量测定.pdf.pdf

中华中医药44学刊第26卷第1期2008年1月中华中医药学刊CHINESEARCHIVESOFTRADIT10NALC:HINESEMEDICINEVo】.,寿文虹,赵建英(金华市药品检验所,浙江金华321000)摘要:目的:建立柑橘黄酮中总黄酮及橙皮苷的含量测定方法。方法:采用分光光度法,以芦丁为对照品,测定总黄酮的含量,检测波长为500nm。采用高效液相色谱法测定橙皮苷的含量,色谱柱:AgilentZORBAXC,(150mm×,);%磷酸(22:78)为流动相,检测波长为284nm,流速1mL/min。结果:—(r=)的浓度范围内线性关系良好,%。—(r=)范围内线性关系良好,%。结论:两种方法均准确、重现性好,适用于柑橘黄酮的质量控制。关键词:柑橘黄酮;橙皮苷;紫外分光光度法;高效液相色谱法中图分类号:11284文献标识码:A文章编号:1673—7717(2008)O1一OO44—02DeterminationofTotalFlavonoidsandHesperidininCitrusFlavonoidsLUBei,SHOUWen—hong,ZHAOJian-ying(JinhuaInstituteforDrugControl,Jinhua321000,Zhejiang,China)Abstract:Objective:ToestablishmethodsforthedeterminationoftotalflavonoidsandhesperidininCitrusFla—:,;AXC18(150ram×,5m)—cludedthemobilephaseconsistingofacetonitrile—%phosphoricacidwatersolution(22:78)~:—(r=)w$%.Thesameresultwasobtainedwhiledetermini