,10(2:0098−0101doi:.-Li,LIYan-Jing,CHENJun,LIPing*KeyLaboratoryofModernChineseMedicines,MinistryofEducation,DepartmentofPharmacognosy,ChinaPharmaceuticalUniversity,Nanjing210009,[ABSTRACT]AIM::D-101porouspolymerresincolumn,normal-phasesilicagelcolumnchromatography,revised-phasemediumpressurecolumnchromatography,SephadexLH-20andprep-HPLCwereusedfortheseparation,-:FivesaponinswereisolatedandelucidatedasachyranthosideC(1,achyranthosideD(2,chikusetsusaponinⅣ(3,ginsenosideRo(4andzingibrosideR1(,pound3wasreportedfromthefamilyofAmaranthaceaeforthefirsttime.[KEYWORDS]Achyranthesbidentata;Chemicalconstituents;Triterpenoidsaponins[umber][Documentcode]A[ArticleID]1672-3651(201202-0098-041IntroductionTherootofAchyranthesebidentataBl.,usuallycalledHuainiuxiinChinese,monlyusedmedicinalherbprescribedbypractitionersoftraditionalChinesemedicineforthetreatmentofosteodyniaoflumbarandknees,,diminishinflammation,exciteuterus,decreasebloodglucose,reducebloodfat,rein-forceimmunologicalfunction,delaysenilityandsoon[1].Inordertofurtherinvestigatethebioactiveconstituentsinthisherbforestimatingitsquality,westudiedthechemicalcon--ranthosideC(1,achyranthosideD(2,chikusetsusaponinⅣ(3,ginsenosideRo(4andzingibrosideR1(pound1obtainedfromnaturalsourceforthefirsttime,-4micro-melting[Receivedon]21-Mar.-2011[Researchfunding]ThisprojectwassupportedbyProgramforChangjiangScholarsandInnovativeResearchTeaminUniver
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