白藜芦醇对豚鼠心室肌细胞L.doc

白藜芦醇对豚鼠心室肌细胞L    【关键词】 白藜芦醇;全细胞膜片钳;钙电流 EffectsofresveratrolonLtypecalciumcurrentinisolatedventricularmyocytesofguineapig 【Abstract】AIM:ToinvestigatetheeffectsofresveratrolontheLtypecalciumcurrent(ICaL)inisolatedventricularmyocytesof :,50,100μmol/:①ResveratrolreducedtheICaLinaconcentrationdependentmanner,butithadnoeffectonIVshapeofICaL.②ResveratrolmarkedlyshiftedthesteadystateactivationcurveofICaLtotheright, andV1/2valueincreasedfrom(-±)mVincontrolto(-±)mVinthepresenceofresveratrol(n=7,P=),theκvaluefrom(±)to(±)(n=7,P=).③InactivationcurveofICaLwasnotaffectedsignificantly.④ThetimeconstantofreactivationofICaL wasreducedfrom(±)msto(±)ms(n=6,P=):ResveratrolinhibitedtheLtypecalciumchannel,mainlyactingontheactivityandrecoveryprocesses. 【Keywords】resveratrol;wholecellpatchclamp;calciumcurrent 【摘要】目的:研究白藜芦醇对正常豚鼠心室肌细胞L型钙电流(ICaL):采用全细胞膜片钳技术,:①白藜芦醇呈浓度依赖性阻断ICaL,但对激活电位、峰电位、反转电位均没有影响;②白藜芦醇使ICaL稳态激活曲线右移,V1/2由对照的(-±)mV变为(-±)mV(n=7,P=),κ由(±)变为(±)(n=7,P=);③对失活曲线无影响,不改变ICaL失活特性;④白藜芦醇使ICaL失活后再恢复时间常数τ缩短,从(±)ms变为(±)ms(n=6,P=0