#SupplementaryMaterial(ESI)munications#Thisjournalis©TheRoyalSocietyofChemistry2004Supplementaryinformation:Rapididentificationofnewbacterialalcoholdehydrogenasesfor(R)-and(S)-enantioselectivereductionofß-ketoestersJieZhang,,BernardWitholt,ZhiLi*InstituteofBiotechnology,ETH-Zurich,Honggerberg,CH-8093Zurich,******@)MRspectraweredeterminedat300or400and75or100MHz,respectively,(APCI).BioconversionwasanalyzedbyGCorHPLC-)onditionsGC-pound2forscreening,column:Lipodex-A,25m×;temperatureprogram:40oCto120oCat5oC/min,increasethetemperatureto170oCat45oC/min;retentiontime:(S)-(R)--pound2forpreparativebiotransformation,column:Chirasil-DexCB(Chrompack),30m×;temperatureprogram:50oCto160oCat10oC/min;retentiontime:(S)-(R)--pound4forscreeningandpreparativetransformation,column:ChiralcelOB-H,250mm×;eluent:n-hexane/2-propanol75/25;flowrate:;UVdetection:210nm;retentiontime:(+)-(-)--analysisofconversionof1to2,Column:ChrompackOptima-5column(25m×),temperatureprogram:50oCfor10min,increaseto280oCatarateof10oC/min,keep
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