实验常用试剂缓冲液配制方法.doc

实验常用试剂、缓冲液的配制方法1、1MTris-HCl           □组份浓度1MTris-HCl(,,)   □配制量1L    □。                         ,充分搅拌溶解。                         。                                                       pH值                     浓HCl                                                                                 约70mL                                                                               约60mL                                                                               约42mL                       。                        ,室温保存。                           注意:应使溶液冷却至室温后再调定pH值,因为Tris溶液的pH值随温度的变化差很大,温度每升高1℃,。 2、-HCl    □-HCl   ()           □配制量1L        □。                          ,充分搅拌溶解。                          。                            。                        ,室温保存。       注意:应使溶液冷却至室温后再调定pH值,因为Tris溶液的pH值随温度的变化差异很大,温度每升高1℃,。 3、10×TEBuffer       □组份浓度100mMTris-HCl,10mMEDTA(,,) □配制量1L □,置于1L烧杯中。                              1MTris-HClBuffer(,,)100mL                            500mMEDTA()20mL                      ,均匀混合。                    ,高温高压灭菌。                    。4、3M醋酸钠     □组份浓度3M醋酸钠   ()          □配制量100mL   □•3H2O置于100~200mL烧杯中,加入约40mL的去离子水搅拌溶解。                          。                      。                         ,室温保存。5、PBSBuffer         □组份浓度137mMNaCl,,10mMNa2HPO4,2mMKH2PO4                               □配制量1L□,置于1L烧杯中。                                                     NaCl                   8g                                                     KCl                                                                          Na2HPO4