大叶白麻叶化学成分的研究.pdf.pdf

分类号密级大叶白麻叶化学成分的研究 Y 902928 研冗生: 煞篮莲指导教师: 尘直出数攫合作指导教师: 巾请学位门类级别专业名称研究乃向抖堂堂』Z 蕴塑丝堂主苎丝主缝佥曲监筮所在学院: 麴堂瞳 2006年5月22同单位代码: 学 10114 2003097 山西医科大学颤j1学位沦文摘要大叶白麻(Pocynum hendersonii(‘)Woodson)为夹竹桃科白麻属的多年生宿根性草本植物,在我国,主要分布于青海、新疆和甘肃等省区。近年来的药理研究表明:大叶白麻叶的醇提物具有抑制血小板聚集、延缓衰老和降血压、降血脂和改善心脏功能的作用。本课题对大叶白麻叶的化学成分进行了提取、分离、纯化和结构的鉴定。实验用的原药材由山西亚宝制药厂提供,产地为新疆格尔木,经山西医科大学药学院生药教研室主任高建平教授鉴定为夹竹桃科白麻属的大叶白麻叶。本文采用75%乙醇作为提取溶剂,通过渗漉法从原药材中提取得到粗提物,并用减压法回收溶剂。采用石油醚、氯仿、乙酸乙酯和正丁醇依次萃取的方法(即系统分离法)对粗提物进行初步分离,将其分成五个部位,然后用硅胶双向柱层析(石油醚一乙酸乙酯系统梯度洗脱)和聚酰胺干法减压柱层析(乙醇一水和甲醇一乙酸乙酯系统梯度洗脱)分别对石油醚部位和乙酸乙酯部位进行进一步分离。采用硅胶湿法柱层析(石油醚一乙酸乙酯、石油醚一丙酮系统梯度洗脱)和重结晶的方法对石油醚部位进行纯化;采用Sephadex (无水甲醇和氯仿一甲醇系统洗脱)和重结晶的方法对乙酸乙酯部位进行纯化。通过薄层检测和熔点测定等方法和手段初步判定化合物的纯度,然后通过各种显色反应和IR判断化合物中特征官能团的类型和骨架类型,MR等波谱方法对化合物的结构进行鉴定。本课题从大叶白麻叶中分离得到1 5个化合物,共鉴定了 12个单体化合物,其中包括2个三萜类化合物,9个黄酮类化合物和1个酚酸,即:D-谷 f{{醇、羽扇豆醇棕榈酸酯、槲皮素、异槲皮苷、、芦丁、三奈酚、三叶豆苷、紫云英苷、异鼠李素3-O一葡萄糖苷、北美圣草素7-。其中, 三奈酚、三叶豆苷、没食子酸和北美圣草素7-。关键词:大叶白麻,化学成分,黄酮,分离纯化,结构鉴定山曲医科大学硕士学位论文 ABSTRACT Pocynum hendersonii( Woodson is aperennial herbaceous plant from Poacynum Baill inApoacynaeease,and mostly grows inQinghai,Xinjiang,Xizhang andotherprovinces of has been reported that the alcohol extract from the leaves of Pocynum hendersonii(Hook.￡1 Woodson exerts various pharmacological effectsincluding antihematoblast collectiveand antisenescent and antihypertensive and reducing serous lipoprotein as well as improving cordis function chemical constituents from leaves of Pocynum hendersonii(Hook.￡)Woodson were extracted,isolated,purified andfinally identifiedinthe experiment. The leaves ofPocTnum hendersonii(Hook.￡)Woodson,a kindofcrudematerials usedinthe experiment,were supplied by Yabao pharmacy factory inShanxi province,and were collected from Geermu region inXinjiang province,the identification oftheexperimental materials WaS performed by professor Jianping Gao,the dbector of crude drug laboratory from the Pharmaceutic School,Shanxi MedicalUniversity. The primary extrac