外文文献.doc

In vitro effect of lemon and orange juices on calcium oxalate Crystallization Received: 10 March 2007 / Accepted: 10 July 2007 / Published online: 25 August 2007 Springer Science +Business Media . 2007 Abstract Aim In recent years significant progress has been made in identifying and quantization physics-chemical processes involved in urinary stone formation. The ability of urine to inhibit calcium oxalate crystallization is an important mechanism against stone formation. Dietary factors appear to affect the ability of urine to inhibit calcium oxalate crystallization. These factors encouraged us to study the effects of the lemon and orange juices on calcium oxalate crystallization in vitro. Material and methods The nucleation and aggregation of calcium oxalate monohydrate crystals were studied using turbid metric 30-min time course measurements of optic density at620 nm after mixing solutions containing calcium chloride and sodium oxalate at37℃, pH=. The formation of crystals is induced by the addition of the oxalate and calcium solution. The effects on calcium oxalate crystal growth of trisodium citrate, lemon and orange juices were examined. The effects of lemon and orange juices were evaluated by the addition of50 mlof juices. The optical density is measured at physiological conditions. The maximum increase of optic density with time, termed SN, reflects maximum rate o