毕业设计(论文) 201 2 届题目穿心莲内酯的提取工艺研究专业制药工程学生姓名方梅芬学号08 0923 12 指导教师尹莉论文字数 10319 字完成日期 201 2年5月湖州师范学院教务处印制湖州师范学院学士学位论文穿心莲内酯的提取工艺研究 0809231 2方梅芬(湖州师范学院生命科学学院,湖州 313000) 摘要: 在单因素试验的基础上, 采用正交试验法优选穿心莲内酯超声波提取法最佳提取条件。以穿心莲茎干粉末为原料,改变超声振荡功率、时间、料液比、不同溶剂、反应温度等单因素变量,提取液经配制后测量吸光度值,选定三水平后进行正交试验。以穿心莲内酯对照品制备标准液, 计算回归方程为: Y=- ,R 2 = 。结果表明, 超声波提取的最佳工艺条件为:在 60℃下,以乙酸乙酯为溶剂,料液比为 1:30 ,功率为 140w ,超声提取 60min , 测得穿心莲提取液的吸光度为 。关键词: 穿心莲内酯; 超声波; 提取 E xtraction P rocess of Andrographolide 0809231 2 FANG Meifen ( School of Life Science , Huzhou Teachers College , Huzhou 313000 ) Abstract: On the basis of one-factor experiment, orthogonal experiment method was employed for the optimization of ultrasonic extraction of Andrographis paniculta lactone. To stem powder andrographis as raw materials, change the s ingle facto r variables of ultrasonic oscillator power, the oscillator time, the solid-liquid ratio , the different solvent and the reaction temperature ,e xtrat after configuration measuring absorbance , and then selected three levels before orthogonal experiment . With andrographis paniculta lactone reference substance preparation standard fluid, calculate a regression equation for: Y=1 3. 7828 1x- 74R 2= 692 .T he optimum conditions of ultrasonic extraction of Andrographis paniculta lactone as follows: in 60℃, use ethyl acetate , the solid-liquid ratio is 1: 30, ultrasonic oscillator power is 140 w, and ultrasonic extraction 60 min. measuring absorbance Andrographis paniculta e xtrat is . Key words: andrographis paniculta lactone; Ultrasonic; Extracted; absorbance 湖州师范学院学士学位论文目录 1引言.............................................................................................................................. 1 穿心莲简介............................................................................................................ 1 穿心莲 穿心莲内酯的药理活性 超声提取概述....................................................................................................
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