磁性纳米粒子制备、表面修饰及对还原型谷胱甘肽的吸附的研究.pdf

华东师范大学2007届硕士学位论文摘要磁性纳米粒子以其特殊的磁性能,在生物医学领域如固定化酶、靶向药物、细胞和DNA分离等方面有广泛的应用前景。这些应用要求磁性纳米粒子具有优异的分散性、均一的尺寸、稳定的化学性质和良好的生物相容性,因此研究单分散磁性纳米粒子的制备、表面修饰及对生物药物分子的吸附组装具有十分重要的意义。本文就这三个方面开展了如下的研究工作: 1高温油相法制备单分散磁性纳米粒子研究以油酸钠、三氯化铁、油酸、液体石蜡等作为原料,采用高温油相法制备磁性纳米粒子。结果表明:黑色磁性纳米粒子晶相是Fe304,形貌为球形、粒径在 30rim左右,在油相中有较好的分散性。 2油酸钠对油相法制备的Fe304纳米粒子表面改性研究采用表面活性剂油酸钠对高温油相法制备的Fc304磁性纳米粒子进行表面改性,并研究温度、pH值、油酸钠的浓度、磁性粒子在油相中的含量等实验条件对改性结果的影响。结果表明:该方法可以有效地将磁性粒子从油相中转移到水相中,最高转移率达到86%;改性后Fe304粒子表面是亲水性的,且在水相中含量较低时,能够稳定分散较长时间。 3核壳结构SiOz/丫-Fe203纳米复合粒子制备及对还原型谷胱甘肽吸附研究采用反相微乳液法制备Si02/7-Fe203纳米复合粒子,该复合粒子具有核壳结构,良好的分散性,-1。还原型谷胱甘肽在该复合粒子上具有较大的吸附量,是一个多层吸附过程;热力学计算说明还原型谷胱甘肽在核壳结构S102/3'一Fe203磁性纳米复合粒子上的吸附是一个自发的、熵增的、吸热过程。关键词:高温油相法,单分散Fe304纳米粒子,表面修饰,核一壳结构, Si02/v—Fe203磁性复合粒子,还原型谷胱甘肽,吸附华东师范大学2007届硕士学位论文 Abstract ic nanoparticles have high potential applications inbiomedical fields such asenzyme immobilization,dmg targeting,cell and DNA those applications,it demands ic nanoparticles having good dispersity,uniform particle size,stable chemical property,and excellent itis significant to study preparation ofmonodisperse ic nanoparticles,surface modification ic nanoparticles andadsorption ofbiological molecule on ic have done some research works on thethreefollowing items: Firstly,sodium oleate、ferric chloride、oleic acidandliquidparaffin asreaction materials were used toprepare ic nanoparticles showed thatthe main crystal ofsample was sizeof Fe304 nanoparticles with spherical morphology isabout 30rim and ic panicles can disperse anic solution. Secondly,sodium oleate was used assurfacemodificationagent tomodify the surface of Fe304 nanoparticles prepared inhigh anic solution. Parameters oftheexperiment such astemperature、pH value、concentration of sodium oleate and content icpanicles anic phase were investigated and resultsshowed thatthemethod was effective intransferring Fe304 nanoparticles anic phase toaqueous solutionand themaximum ratioofFe304na