“柴胡-黄芩”药对保肝作用有效部位的研究(Ⅱ).pdf

2008年第14卷第3期中国中医基础医学杂志 chinese Journal Basic Medici眦in 1hditioml chinese Medicine 79 【实验研究】“”药对保肝作用有效部位的研究(Ⅱ)。许刚,谢鸣△(北京中医药大学基础医学院,北京 100029) 摘要:目的:进一步探查柴胡一黄芩药对水提液保肝作用有效部位。方法:采用溶剂萃取法和乙醇沉淀法,将柴胡一黄芩药对水提液的有效部位c、D进一步分为I、Ⅱ、Ⅲ、Ⅳ4个部位,以血清 ALT、AsT为指标,ld急性肝损伤模型的保护作用,分析确定最佳配伍并对其进行效用验证。结果:正交分析表明,部位I和Ⅲ均能不同程度降低模型大鼠血清 ALT和AsT,其中部位I的作用有显著性差异(JP<),部位最佳配伍为I+Ⅲ;重复验证实验表明,与模型组相比,I+Ⅲ组大鼠血清ALT与AST均显著降低(P<),其效应强度与柴胡一黄芩药对水提液相当。结论:部位I、Ⅲ是柴胡一黄芩药对水提液中保肝作用的主要部位。关键词:药对;l4;保肝作用;有效部位中图分类号: 文献标识码:B 文章编号:(2008) Study on Effect ofEffective Parts from Chai-hu MatchedH岫ng—qin XU Gang,XIE Min96 (coZ如酽矿B∞ic胁批妇,&咖,lg‰泐瑙毋旷7砌咖Z吼i嬲e讹did船,&驴ng 100029,吼iM) : ObjectiVe: T0 funher explore the effective fraction ofthe hepatopmtective effect inthe decoction ofBupleutrum chinese—Scutellaria baicalensis Geor百he南al :1he effectiVefractionC and D inthedecoction ofBupleutmm chinese—ScutellaIia baicalensis i herbal pair were furtherdivided intofour fhctions, which were I、Ⅱ、Ⅲ、and Ⅳ,by using methods《solvent extraction and alcohol onhogonal design was used on acute h印aticinjury model induced l4 in mts investigate thehepatoprotective efkcts ofthefourf妇ctionsbv measuring the1evelofAUl and AST insemm. The bination dete byanalysis was validated inanother experiment. Results: According to analysis oforth090nal desigIl,f王actionIandⅢcould d