- 1 - Investigation on the Interaction of Glutathione and Glutathione-S-Transferase with the BSA-bounded biochip Using Surface Plasmon Resonance Lihua CHEN College of chemistry and molecular engi neer, Qingdao University of Science and Technology. Qingdao. 266042, China E-mail:******@qust. Abstract :A chip was modified with bovine seru m albumin (BSA), then interaction between glutathione (GSH) immobilized on the top of BSA and glutathione-S-transferase (GST) was ex amined using surface plasmon resonance (SPR). The SPR results showed that BSA- modified chip was effective not only in binding the target proteins but also in s uppressing the nonspecific binding (NSB) of proteins. Keywords: Surface plasmon resonance (SPR), bovine serum albumin (BSA), glutathione (GSH), glutat hione-S-transferase (GST). Recently, surface plasmon re sonance (SPR) e more and more popular without the need of the label technology. 1-3 However, sometimes, a number of experimental plicate the final biosensor analysis. 4-7 The utilization of a reference surface can dramatically improve the quality of the binding data by correcting artifacts, such as matrix eff ects, nonspecific binding and baseline drift. 1, 5, 8-10 To design a suitable reference surface, th e nonspecific binding of proteins to chip must be resolved first. By far two methods have been employed. One is to modify t
