该论文来源于网络，本站转载的论文均是优质论文，供学习和研究使用，文中立场与本网站无关，版权和著作权归原作者所有，如有不愿意被转载的情况，请通知我们删除已转载的信息，如果需要分享，请保留本段说明。 摘 要：目的：探究不同培养条件对哈茨木霉菌抑制串珠镰刀菌能力的影响。方法：采用平板对峙培养法，以抑菌率为指标，研究不同培养时间、碳源、培养基、pH值对哈茨木霉菌抑制串珠镰刀菌能力的影响。结果：不同培养时间、碳源、培养基、pH值对哈茨木霉菌抑制串珠镰刀菌的能力均有影响。培养到第4天时抑菌效果达到最佳，%;不同碳源中，最优为葡萄糖、麦芽糖、甘露醇和乳糖;PDA培养基培养对哈茨木霉菌抑制串珠镰刀菌的生长效果最佳;pH值为5～9时，哈茨木霉菌对串珠镰刀菌抑制率无差异。结论：PDA培养基培养到第4天达到最佳抑制效果，多种碳源及广泛的pH值范围均适合哈茨木霉菌对串珠镰刀菌的生长形成抑制。 关键词：哈茨木霉菌;串珠镰刀菌;平板对峙培养法 Abstract： To investigate the effects of different culture conditions on the ability of Trichoderma harzianum to inhibit Fusarium moniliforme. The effect of different culture time， carbon source， culture medium and pH value on the ability of Rhizoctonia solani to inhibit the ability of Fusarium moniliforme was studied by using platelet confinement culture method and antibacterial rate as an index. Different culture time， carbon source， culture medium and pH value had an effect on the ability of Trichoderma harzianum to inhibit Fusarium moniliforme. The antibacterial effect was the best at the 4th day， and the antibacterial rate was %. Among the different carbon sources， best for glucose， maltose， mannitol and lactose; PDA culture medium is the best effect on Trichoderma harzianum to inhibit Fusarium moniliforme; pH value of 5 to 9 when Trichoderma harzianum on the inhibition rate of Fusarium moniliforme no ： PDA culture medium reached the best inhibitory effect on the 4th day， and a variety of carbon sources and extensive pH range were suitable for the inhibition of the growth of Fusarium moniliforme. Key words： Trichoderma harzianum; Fusarium moniliforme; Plate culture method 串珠鐮刀菌可引起植物茎腐、根腐[1]、穗腐[2]等症状，使植株生长不良，花梗易折断，最终倒伏。在玉米生长过程中，由多种病原菌单独或复合侵染造成根系和茎基腐烂的茎基腐病，俗称根腐病，是玉米产区一类重大病害。不同年代、不同地区，该病害的发病率略有不同，一般年份发病率在10%～20%，严重年份高达80%～90%，产量受损，严重者甚至颗粒无收[3-4]。一旦发生茎基腐病，玉米果穗与籽粒均受到危害，被侵染的果穗局部或全部被霉层覆盖，霉层呈现粉褐色、红色、灰黑色及黄绿色。发病植株的果穗失去光泽，颗粒不够饱满或瘪空腐烂等。该
