实验四 线虫的分离.ppt

实验四线虫的分离
Some Procedures for Collecting and Preparing Nematodes For Study.
(Note: These procedures may be similar to or modified from those used by other workers.)
1. Materials
1 bucket, 20 mesh sieve, 60 mesh sieve, 325 mesh sieve, and stand to hold sieves.
2. Procedure
Stack the 3 sieves (be sure to wash them, they work better wet!), one on top of the other from top to bottom, 20 mesh, 60 mesh and 325 mesh. Place on stand. Run water through the sieves to be sure they are wet and the water runs freely through them.
Place a soil sample (approximately 250 ml) into bucket. Stir with hand and break up clumps of soil while adding tap water to bucket (approx. 3/4 full).
When solution is uniform, wait 30 seconds for some of the heavy sediment to settle, lift bucket gently and pour through the stacked sieves (20, 60, 325) at one time (leaving the settled sediment in the bottom of the bucket).
Discard the 20 mesh sieve. Then collect cysts and large eel shaped forms from the 60 mesh sieve by backwashing into a 250 ml beaker. Collect the other eel-shaped forms by the same method and backwash the 325 mesh sieve into a separate 250 ml beaker.
After the beakers have settled for about ½ an hour, decant the clear water from the top and put the settled sediment at the bottom of the beaker into a watch glass for further examination.
To save nematodes for further study kill and fix ext