mg132抑制肝癌细胞bel-7404生长的机制研究.pdf

MG132 !"#$%Bel7404 &'()!*+ * ! 1) "# 1) $%& 2)'() 1)*+, 1) -. 1) /0 1) 123 1)** ( !"#$%&'(%%)*+,-%.&/01234*563100187 89(%):;&'(%23)&/<%.=>&/%234*:;200031) ,-MG132(Z-Leu-leu-leu-CHO)?***@ABCDEFGH*IJKLFGBCDEMN*OPFGQR-BCDEST4U VWBCXYZ*[V=>\] ^_23MG132FG`a=>Bel-7404&bWcG defghijiklmji*S nopqrstuvwxqrstHoechst33342yztMTT{|tAnnexin /PI}~=>1tWesternblot {|MG132 !Bel-7404=>W"#%$<t=>%X&'($<t)*+",t\]-E",t=>.M/t=>\]01t\]2 )*3456878BCX9:W;< =>q?*******@AqFGBel-7404=>&b SnBC%X&'(DM Caspase-12*EJSnFGE56HIBcl-2/Bax01*JK=>zRcWLM*NOPJDMQRSTCaspase-3*UV PARP*[V=>\] gl*MG132J[VBeclin1kLC3BW:H*JWBel-7404=>X&)**JYuvwsQZ[ \)*+",:]=>9A*MG132^eBel-7404=>_2N`=>abNc]56d=>\]k)* ./0BCDEFGH*`a=>*\]*)**%X&TD 12345 Q2*Q7 DOI: . &6718&669:; ProgressinBiochemistryandBiophysics 2010,37(6):627~634 *9e)f(%ghijkl(30800093)k !mn8on%(p Mgh(SWYX0809). **Sqrst. Tel:0571-86843181,E-mail:zhouxiumei824@ uvwxd2009-12-29*yzwxd2010-03-24 =>&.W8{Y|)}BCX~,kZW 1 QR-BCDE56(ubiquitinproteasome pathway*UPP)?&/E%BCX !NYZWn "56o@ [1]*#"^e|=>%$%x&WHI BCk'$%x(bWBCX*)2@*=+, -t./0z12WBCX [2] 3na?4A5B C)67{"~r=89QR(ubiquitin)x*: QR<(ubiquitination);*<=>\26SBCDE :?@<YZ*A?26SBCDE19SBCDE *F;QR<5BCGQR<tHpI/kJ D*KK20SBCDWLM=+%?8NVO* P;@<Q/*LMR3!2298SW&STU [3]. >Xt=>\ ]t=>YxtDNAZ[t\]2^_TD`a V78 [4-5]*`cdWX&78*ef .UPPg8WD?hi?j/klWx5m BCDEnoYZ=>%4A5BC* A=>q@ rTe3s,NFGHt$3DVD uMN0FGQRBCDEST