Lecture 20 Cell- and Tissue-based biosensors.pdf

Molecular Principles of Biomaterials Spring 2003

Lecture 20: Cell- and Tissue-based biosensors


Last time: detection methods
Surface plasmon resonance biosensors


Today: cell- and tissue-based sensors
Primary transducers and biosensor design with living cells
microphysiometer


Reading: . Pancrazio et al., ‘Development and application of cell-based biosensors,’ Ann.
Biomed. Eng. 27, 697-711 (1999)


Cell-based biosensors1-6

General concepts

ƒ Why cell-based biosensors?
o Known ultrasensitivity of cells:
ƒ Olfactory neurons respond to single odorant molecules
ƒ Retinal neurons triggered by single photons
ƒ T cells triggered by single antigenic peptides7


¥Potential for single-
molecule sensitivity
-retinal neurons triggered by
single photons
-olfactory neurons detect single
odorant molecules
-T cell recognition of foreign
peptide (shown at right)
¥Cellular machinery
maintains physiological
status of receptors
involved in detection
¥Complex ÔevaluationÕ of
agents Calcium signaling
Error! (Irvine et al. 2002)

o Ability to ‘integrate’ cellular or tissue response pounds
ƒ Detect functionality pound in addition to its chemical presence
• . tell the difference between a dead and live virus
Lecture 20 – Biosensors 1 of 8
Molecular Prin