HPLC 法测定清火栀麦片中穿心莲内酯、脱水穿心莲内酯的含量酯及脱水穿心莲内酯测定的 HPLC 法。方法外标法, DiamonsilTMC18 色谱柱(150mm × ,5μm);流动相:甲醇-% 冰醋酸水溶液( 46:64);检测波长 225nm 和250nm ;流速 1ml/min 。结果穿心莲内酯的线性范围为 ~ μg(r= ),平均回收率为 % ,RSD=% (n=5) ;脱水穿心莲内酯的线性范围为 ~ μg(r=) ,平均回收率为 % , RSD=% (n=5) 。结论方法准确、灵敏,可作为清火栀麦片中穿心莲内酯及脱水穿心莲内酯的定量分析方法。【关键词】片剂;穿心莲内酯;脱水穿心莲内酯;高效液相色谱【 Abstract 】 Objective Todetermine the content of andrographolide 、dehydroandrographolide inQinghuozhimai tablets by HPLC. Methods Anexternal standard method byHPLC with DiamonsilTMC18 column asfixed phase and methanol-%HAC(46:64) as mobile phase was adopted. The detection wavelength was 225nm 、250nm and the flow rate was . Results The linear range for andrographolide was ~ μg(r=).The average recovery oftablets sample was % and RSD was % (n=5);the linear range for dehydroandrographolide was ~ μg(r=). The average recovery oftablets sample was % and RSD was % (n=5). Conclusion The method issimple and accurate ,which can be adoptable for quantitative analysis ofandrographolide 、 dehydroandrographolide ofQinghuozhimai tablets. 【Key words 】tablets; andrographolide;dehydroandrographolide;HPLC 清火栀麦片[ 1]是由穿心莲[ 2]、栀子、麦冬三味药经提取精制而成,具有清热解毒,凉血消肿等功效,主要用于咽喉肿痛、发热、牙痛、目赤等。但其质量标准无两种有效成分的同时测定方法[ 3~5]。为完善质量控制标准,本文选择采用穿心莲内酯和脱水穿心莲内酯作为测定对象,建立了用 HPLC 同时测定清火栀麦片中穿心莲内酯、脱水穿心莲内酯的方法。 1 仪器、试剂
