个人收集整理 勿做商业用途 个人收集整理 勿做商业用途 个人收集整理 勿做商业用途 ABSTRACT This article use defatted wheat germ as raw material to prepare antioxidative peptides。 The conditions of hydrolytic pretreatm— ent of wheat germ protein were investigated. The results are as follows: The conditions of hydrolytic pretreatment of wheat germ protein. The experiment was conducted to study the process parameters on enzymatic hydrolysis and antioxidant activity of hydrolysates by five protease. The results showed that the neutral protease was the best single enzyme， the neutral protease and the alkaline protease were the best double-enzyme. In addition， single enzyme had a better enzyme hydrolysis result by comparison with the double-enzyme。 The optimum hydrolytic conditions were： substrate concentration 4％, neutral protease dosage 6000u/g, temperature 50℃, and pH value . Under this conditions, the antioxidant activity of hydrolysates attained maximum when hydrolysis time 270 min, and the DH was 36。70%，
