线栓法建立局灶性脑缺血模型的方法与探讨.doc

线栓法建立局灶性脑缺血模型的方法与探讨.doc线栓法建立局灶性脑缺血模型的方法与探讨
［摘要］目的探讨成功建立大鼠局灶性脑缺血模型的有效方法及影 响因素。方法将大鼠分为250〜300 g和350〜400 g两个体重组。为观 察禁食对造模的影响，250-300 g体重组又分为对照组(n=10)和禁食组 (n=10)；为观察栓线直径对造模的影响，350〜400 g体重组又分为细栓 线组(n=10)和粗栓线组(n=10)。栓线经颈总动脉、颈内动脉进入大脑 中动脉的起始部位阻断大脑中动脉的血流。术后通过神经功能缺损评分和 氯化三苯基四氮呼(TTC)染色对该模型进行评价。结果在250〜300 g 体重组，造模成功率高，禁食组死亡率(%)明显低于对照组(%) (P < 0. 05)；在350〜400 g体重组，死亡率低，粗栓线组造模成功率(80%) 明显高于细栓线组(20%) (P < )。结论术前禁食可降低动物死亡 率，根据大鼠体重选择相应直径的栓线可提高造模的成功率。
［关键词］栓线法；局灶性脑缺血；禁食；栓线直径
［中图分类号］R363. 1 ［文献标识码］A ［文章编号］1673-7210 (2014)
08 (c) -0033-03
The met hod and explore of est ablishing focal ischemia model with
bolt line
JIA Shuweil XIA Qing2
Department of Physiology , Harbin Medical University ,
Heilongjiang Province, Harbin 150086, China； of Acupuncture, Tianjin Chinese Medical University, Tianjin 300193, China
[Abstract] Objective To investigate the method and influence factors of establishing focal ischemia model of rat successfully. Methods Rats were divided into two groups, 250-300 g group and 350-400 g group. In order to observe the influence of fasting on the model, 250-300 g group was subdivided into two groups, control group (n=10) and fas ting group (n=10) . In order to observe the influence of the bolt line diameter on the model, 350-400 g group was sub-divided into two groups, thin bolt line group (n=10) and bold bolt line group (n=10) . The bolt line entered from a cut of the co