补脾益气中药对脾虚哮喘大鼠环核苷酸水平的影响.pdf

第四届中国中西区结合学会变态反应学求夫会论交汇编蹙009·西安补脾益气中药对脾虚哮喘大鼠环核苷酸水平的影响朱晓明1,杨月妍2,王巍1,朴仁范3,魏庆宇1 (1沈阳军区第202医院变态反应科110003;2辽宁中医研究院药剂科110034;3延边大学中医学院133000) 【摘要】耳的:探讨补脾益气中药对脾虚哮喘大鼠环核苷酸水平的影响。方法:以中医理论为指导,建立了大鼠脾虚哮喘病证结合模型。观察补脾益气法对模型动物支气管肺泡灌洗液(BALF)中细胞总数、嗜酸细胞数(Eos)及BALF上清液中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMF)含量及二者的比值的影响,()对BALF细胞孵育液中cAMP、cGMP含量及二者比值的影响。结果:与正常对照组比较,脾虚哮喘动物和哮喘动物均表现为Tot、Eos升高,cAMP含量降低,cGMP含量升高,cAMPIcGMP比值降低。脾虚哮喘动物的变化更为明显。补脾益气中药对脾虚哮喘和哮喘动物均可降低其Tot、Eos降低cGMP含量,升高cAMP含量和cAMPIcGMP比值。BALF细胞孵育液中,,升高cAMP含量和cAMPIcGMP比值。结论:补脾益气中药可升高c-镰伊含量,降低cGMP含量,纠正cAMPIcGMF比值。。【关键词】哮喘;补脾益气;环磷酸腺苷;环磷酸鸟苷;动物模型 The influnce ofthemothod of‘‘reinforcing thespleen toreplenish QI’’on the levelofcyclic nucleoside ofthe ratasthmatic models with spleen-deficiency ZHUXiao-mingl,YANG Yue-yan2,WANG Weil,PIAORen-fard,WEI Qing-yul. 1Departlnent ofAllergy Diagnosis, Hospital ofShenyang MilitaryArea, ofmedicament,Liaoning Provincial InstituteofTCM, University ofTCM 133000 Abstract Objective:To explore the effectsof themethod of‘'reinforcing thespleen replenish QI”on the contents ofcAMP and cGMP of the ratasthmatic models withspleen—deficiencyMethod:According thetheory ofTraditional Chinese Medicine(TCM),the rat asthmatic models耐thspleen-deficiency WOI'O setupby themethods ofsetting up animalmodel ofspleen—deficiency inTCM and bronchial