1 链霉菌实验室操作手册第一章培养基抗生素生长因子.......................................... 3 常用抗生素及其使用浓度........................................................................................................ 3 LB ( Luria-Bertani )培养基................................................................................................... 3 LA ............................................................................................................................................... 3 基本培养基( MM ) ................................................................................................................. 3 R5 ( 1L ) 链霉菌原生质体转化时用....................................................................................... 4 YEME( 酵母膏- 麦芽膏培养基) 1L (液体培养链霉菌) ...................................................... 4 TSBY ( 1L ) (液体培养链霉菌) ......................................................................................... 4 MS 培养基.................................................................................................................................. 5 2CMY 培养基(用于井岗的接合转移) ................................................................................ 5 YMS 培养基( 阿维, 井岗产孢) .......................................................................................... 5 YD 培养基.................................................................................................................................. 5 生长因子补充物的使用浓度.................................................................................................... 5 第二章 DNA 基本操作.............................................................. 7 大肠杆菌质粒的抽提................................................................................................................ 7 酶连反应..................................................................................................................................... 7 DNA 片段凝胶回收................................................................................................................... 7 DNA 纯化.............
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